NDLI: Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein

Content Provider	PubMed Central
Author	Wagner, Eric J. Ospina, Jason K. Hu, Yue Dundr, Miroslav Matera, A. Gregory Marzluff, William F.
Copyright Year	2006
Abstract	Formation of the 3′ end of replication-dependent histone mRNAs is most robust during S phase and is mediated by both the stem–loop binding protein (SLBP) and the U7 snRNP. We previously identified a 100-kDa zinc finger protein (ZFP100) as a component of U7 snRNP that interacts with the SLBP/pre-mRNA complex. Here, we show that myc- or GFP-tagged ZFP100 overexpressed after transfection is concentrated in Cajal bodies (CBs), and unlike components of the spliceosomal snRNPs, photobleaching experiments demonstrate that ZFP100 is stably associated with CBs. Of the 18 zinc fingers contained within ZFP100, the region encompassing fingers 2–6 is sufficient to maintain CB localization. Zn fingers 5–10 are required for maximal binding of ZFP100 to a 20-amino-acid region of Lsm11, a U7 snRNP core protein. Expression of ZFP100 stimulates histone mRNA processing in vivo, assayed by activation of a reporter gene that encodes a GFP mRNA ending in a histone 3′ end. Importantly, the domain that is required for CB localization and Lsm11 binding is also sufficient to stimulate histone pre-mRNA processing in vivo. Comparisons with other mammalian ZFP100 orthologs show that the central Zn fingers sufficient for in vivo activity are most highly conserved, whereas the number and sequence of the Zn fingers in the N- and C-terminal domains vary.
Related Links	http://dx.doi.org/10.1261/rna.2606
Ending Page	1218
Page Count	13
Starting Page	1206
File Format	PDF
ISSN	13558382
e-ISSN	14699001
Journal	RNA
Issue Number	7
Volume Number	12
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2006-05-01
Access Restriction	Open
Rights Holder	Cold Spring Harbor Laboratory Press
Subject Keyword	Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology

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