NDLI: Water permeability of Na+–K+–2Cl− cotransporters in mammalian epithelial cells

Content Provider	PubMed Central
Author	Hamann, Steffen Jaime, Herrera-perez José Bundgaard, Magnus Javier, Alvarez-leefmans Francisco Zeuthen, Thomas
Copyright Year	2005
Abstract	Water transport properties of the Na+–K+–2Cl− cotransporter (NKCC) were studied in cultures of pigmented epithelial cells (PE) from the ciliary body of the eye. Here, the membrane that faces upwards contains NKCCs and can be subjected to rapid changes in bathing solution composition and osmolarity. The anatomy of the cultured cell layer was investigated by light and electron microscopy. The transport rate of the cotransporter was determined from the bumetanide-sensitive component of 86Rb+ uptake, and volume changes were derived from quenching of the fluorescent dye calcein. The water permeability (L p) of the membrane was halved by the specific inhibitor bumetanide. The bumetanide-sensitive component of the water transport exhibited apparent saturation at osmotic gradients higher than 200 mosmol l−1. Cell shrinkages produced by NaCl or KCl were smaller than those elicited by equi-osmolar applications of mannitol, indicating reflection coefficients for these salts close to zero. The activation energy of the bumetanide-sensitive component of the L p was 21 kcal mol−1, which is four times higher than that of an aqueous pore. The data suggest that osmotic transport via the cotransporter involves conformational changes of the cotransporter and interaction with Na+, K+ and Cl−. Similar measurements were performed on immortalized cell cultures from the thick ascending limb of the loop of Henle (TALH). Given similar overall transport rates of bumetanide-sensitive 86Rb+, the NKCCs of this tissue did not contribute any bumetanide-sensitive L p. This suggests that the cotransporters of the two tissues are either different isoforms or the same cotransporter but in two different transport modes.
Related Links	http://dx.doi.org/10.1113/jphysiol.2005.093526
Starting Page	123
File Format	PDF
ISSN	14697793
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 1
Volume Number	568
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2005-10-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cotransport of water by the Na+ −K+ −2Cl− cotransporter NKCC1 in mammalian epithelial cells

Cotransport of water by Na+–K+–2Cl− cotransporters expressed in Xenopus oocytes: NKCC1 versus NKCC2

Upregulation of Na(+)-K(+)-2Cl- cotransporter activity in rat parotid acinar cells by muscarinic stimulation.

Activation of ferret erythrocyte Na+–K+–2Cl− cotransport by deoxygenation

Phosphorylation and Transport in the Na-K-2Cl Cotransporters, NKCC1 and NKCC2A, Compared in HEK-293 Cells

Regulation of Na+-K+-2Cl− cotransport in turkey red cells: the role of oxygen tension and protein phosphorylation

Kinase regulation of Na+ −K+ −2Cl− cotransport in primary afferent neurons

Regulation of Na+-K+-2Cl− cotransport by protein phosphorylation in ferret erythrocytes

Stimulation of Na+-K+-2Cl− cotransport by arsenite in ferret erythrocytes

Water permeability of Na+–K+–2Cl− cotransporters in mammalian epithelial cells

Similar Documents

Cotransport of water by the Na+ −K+ −2Cl− cotransporter NKCC1 in mammalian epithelial cells

Cotransport of water by Na+–K+–2Cl− cotransporters expressed in Xenopus oocytes: NKCC1 versus NKCC2

Upregulation of Na(+)-K(+)-2Cl- cotransporter activity in rat parotid acinar cells by muscarinic stimulation.

Activation of ferret erythrocyte Na+–K+–2Cl− cotransport by deoxygenation

Phosphorylation and Transport in the Na-K-2Cl Cotransporters, NKCC1 and NKCC2A, Compared in HEK-293 Cells

Regulation of Na+-K+-2Cl− cotransport in turkey red cells: the role of oxygen tension and protein phosphorylation

Kinase regulation of Na+ −K+ −2Cl− cotransport in primary afferent neurons

Regulation of Na+-K+-2Cl− cotransport by protein phosphorylation in ferret erythrocytes

Stimulation of Na+-K+-2Cl− cotransport by arsenite in ferret erythrocytes

Water permeability of Na+–K+–2Cl− cotransporters in mammalian epithelial cells