NDLI: The structural basis of the increase in isometric force production with temperature in frog skeletal muscle

Content Provider	PubMed Central
Author	Linari, M. Brunello, E. Reconditi, M. Sun, Y-b Panine, P. Narayanan, T. Piazzesi, G. Irving, M. Lombardi, V.
Copyright Year	2005
Abstract	X-ray diffraction patterns were recorded from isolated single fibres of frog skeletal muscle during isometric contraction at temperatures between 0 and 17°C. Isometric force was 43 ± 2% (mean ± s.e.m., n = 10) higher at 17°C than 0°C. The intensity of the first actin layer line increased by 57 ± 18% (n = 5), and the ratio of the intensities of the equatorial 1,1 and 1,0 reflections by 20 ± 7% (n = 10), signalling radial or azimuthal motions of the myosin head domains. The M3 X-ray reflection from the axial repeat of the heads along the filaments was 27 ± 4% more intense at 17°C, suggesting that the heads became more perpendicular to the filaments. The ratio of the intensities of the higher and lower angle peaks of the M3 reflection (R M3) was 0.93 ± 0.02 (n = 5) at 0°C and 0.77 ± 0.02 at 17°C. These peaks are due to interference between the two halves of each myosin filament, and the R M3 decrease shows that heads move towards the midpoint of the myosin filament at the higher temperature. Calculations based on a crystallographic model of the heads indicated that the observed R M3 change corresponds to tilting of their light-chain domains by 9 deg, producing an axial displacement of 1.4 nm, which is equal to that required to strain the actin and myosin filaments under the increased force. We conclude that the higher force generated by skeletal muscle at higher temperature can be accounted for by axial tilting of the myosin heads.
Related Links	http://dx.doi.org/10.1113/jphysiol.2005.089672
Ending Page	469
Page Count	11
Starting Page	459
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 2
Volume Number	567
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2005-09-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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