NDLI: Structural studies on tRNA acceptor stem microhelices: exchange of the discriminator base A73 for G in human tRNALeu switches the acceptor specificity from leucine to serine possibly by decreasing the stability of the terminal G1-C72 base pair.

Please wait, while we are loading the content...

Year: 2016, Volume: 44

Year: 2015, Volume: 43

Year: 2014, Volume: 42

Year: 2013, Volume: 41

Year: 2012, Volume: 40

Year: 2011, Volume: 39

Year: 2010, Volume: 38

Year: 2009, Volume: 37

Year: 2008, Volume: 37

Year: 2008, Volume: 36

Year: 2007, Volume: 36

Year: 2007, Volume: 35

Year: 2006, Volume: 35

Year: 2006, Volume: 34

Year: 2005, Volume: 33

Year: 2004, Volume: 33

Year: 2004, Volume: 32

Year: 2003, Volume: 31

Year: 2002, Volume: 30

Year: 2001, Volume: 29

Year: 2000, Volume: 28

Year: 1999, Volume: 27

Year: 1998, Volume: 26

Year: 1997, Volume: 25

Year: 1997, Volume: 25, Issue: 24

Year: 1997, Volume: 25, Issue: 23

Year: 1997, Volume: 25, Issue: 22

The ups and downs of nucleic acid duplex stability: structure-stability studies on chemically-modified DNA:RNA duplexes.

BplI, a new BcgI-like restriction endonuclease, which recognizes a symmetric sequence.

Oligonucleotide dendrimers: synthesis and use as polylabelled DNA probes.

The ETS family member ERM contains an alpha-helical acidic activation domain that contacts TAFII60.

Functional recognition of fragmented operator sites by R17/MS2 coat protein, a translational repressor.

The motif V of plum pox potyvirus CI RNA helicase is involved in NTP hydrolysis and is essential for virus RNA replication.

TAR RNA decoys inhibit tat-activated HIV-1 transcription after preinitiation complex formation.

Binding of Hoechst 33258 to the TAR RNA of HIV-1. Recognition of a pyrimidine bulge-dependent structure.

The yeast gene YNL292w encodes a pseudouridine synthase (Pus4) catalyzing the formation of psi55 in both mitochondrial and cytoplasmic tRNAs.

New dye-labeled terminators for improved DNA sequencing patterns.

Replication initiation and elongation fork rates within a differentially expressed human multicopy locus in early S phase.

Characterization of multiple alternative RNAs resulting from antisense transcription of the PR264/SC35 splicing factor gene.

NMR determination of the conformational and drug binding properties of the DNA heptamer d(GpCpGpApApGpC) in aqueous solution.

Higher fidelity of RNA-dependent DNA mispair extension by M184V drug-resistant than wild-type reverse transcriptase of human immunodeficiency virus type 1.

High affinity binding of MEF-2C correlates with DNA bending.

The RNA moiety of chick embryo 5-methylcytosine- DNA glycosylase targets DNA demethylation.

Structural studies on tRNA acceptor stem microhelices: exchange of the discriminator base A73 for G in human tRNALeu switches the acceptor specificity from leucine to serine possibly by decreasing the stability of the terminal G1-C72 base pair.

The yeast 8-oxoguanine DNA glycosylase (Ogg1) contains a DNA deoxyribophosphodiesterase (dRpase) activity.

Placement of the alpha-sarcin loop within the 50S subunit: evidence derived using a photolabile oligodeoxynucleotide probe.

RAGA: RNA sequence alignment by genetic algorithm.

2'-Fluoro modified nucleic acids: polymerase-directed synthesis, properties and stability to analysis by matrix-assisted laser desorption/ionization mass spectrometry.

Solution structure of RNA duplexes containing alternating CG base pairs: NMR study of r(CGCGCG)2 and 2'-O-Me(CGCGCG)2 under low salt conditions.

Crystal structure of 2'-O-Me(CGCGCG)2, an RNA duplex at 1.30 A resolution. Hydration pattern of 2'-O-methylated RNA.

Opening of the extraordinarily stable mini-hairpin d(GCGAAGC).

Analysis of the yeast genome: identification of new non-coding and small ORF-containing RNAs.

Statistical modeling and analysis of the LAGLIDADG family of site-specific endonucleases and identification of an intein that encodes a site-specific endonuclease of the HNH family.

Increased DNA binding and sequence discrimination of PNA oligomers containing 2,6-diaminopurine.

Relative stability of triplexes containing different numbers of T.AT and C+.GC triplets.

Non-homologous DNA end joining in plant cells is associated with deletions and filler DNA insertions.

Both the polypyrimidine tract and the 3' splice site function prior to the first step of splicing in fission yeast.

Baculovirus-mediated expression and characterization of the full-length murine DNA methyltransferase.

Peptide nucleic acid-anthraquinone conjugates: strand invasion and photoinduced cleavage of duplex DNA.

RnaViz, a program for the visualisation of RNA secondary structure.

Construction of a semisynthetic antibody library using trinucleotide oligos.

Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.

Adaptor-tagged competitive PCR: a novel method for measuring relative gene expression.

Monitoring the amplification of CATCH, a 3SR based cooperatively coupled isothermal amplification system, by fluorimetric methods.

Isolation of nuclei for chromatin analysis in fission yeast.

Year: 1997, Volume: 25, Issue: 21

Year: 1997, Volume: 25, Issue: 20

Year: 1997, Volume: 25, Issue: 19

Year: 1997, Volume: 25, Issue: 18

Year: 1997, Volume: 25, Issue: 17

Year: 1997, Volume: 25, Issue: 16

Year: 1997, Volume: 25, Issue: 15

Year: 1997, Volume: 25, Issue: 14

Year: 1997, Volume: 25, Issue: 13

Year: 1997, Volume: 25, Issue: 12

Year: 1997, Volume: 25, Issue: 11

Year: 1997, Volume: 25, Issue: 10

Year: 1997, Volume: 25, Issue: 9

Year: 1997, Volume: 25, Issue: 8

Year: 1997, Volume: 25, Issue: 7

Year: 1997, Volume: 25, Issue: 6

Year: 1997, Volume: 25, Issue: 5

Year: 1997, Volume: 25, Issue: 4

Year: 1997, Volume: 25, Issue: 3

Year: 1997, Volume: 25, Issue: 2

Year: 1997, Volume: 25, Issue: 1

Year: 1996, Volume: 24

Year: 1995, Volume: 23

Year: 1994, Volume: 22

Year: 1993, Volume: 21

Year: 1992, Volume: 20

Year: 1991, Volume: 19

Year: 1990, Volume: 18

Year: 1989, Volume: 17

Year: 1988, Volume: 16

Year: 1987, Volume: 15

Year: 1986, Volume: 14

Year: 1985, Volume: 13

Year: 1984, Volume: 12

Year: 1983, Volume: 11

Year: 1982, Volume: 10

Year: 1981, Volume: 9

Year: 1980, Volume: 8

Year: 1979, Volume: 7

Year: 1979, Volume: 6

Year: 1978, Volume: 5

Year: 1977, Volume: 4

Year: 1976, Volume: 3

Year: 1975, Volume: 2

Year: 1974, Volume: 1

Structural studies on tRNA acceptor stem microhelices: exchange of the discriminator base A73 for G in human tRNALeu switches the acceptor specificity from leucine to serine possibly by decreasing the stability of the terminal G1-C72 base pair.

Content Provider	PubMed Central
Author	Metzger, A. U. Heckl, M. Willbold, D. Breitschopf, K. Rajbhandary, U. L. Rösch, P. Gross, H. J.
Abstract	Correct recognition of transfer RNAs (tRNAs) by aminoacyl-tRNA synthetases (aaRS) is crucial to the maintenance of translational fidelity. The discriminator base A73 in human tRNALeuis critical for its specific recognition by the aaRS. Exchanging A73 for G abolishes leucine acceptance and converts it into a serine acceptor in vitro . Two RNA microhelices of 24 nt length that correspond to the tRNALeuacceptor stem and differ only in the discriminator base were synthesized: a wild-type tRNALeumicrohelix, where nt 21 corresponds to the discriminator base position 73, and an A21G mutant microhelix. To investigate whether different identities of both tRNAs are caused by conformational differences, NMR and UV melting experiments were performed on both microhelices. Two-dimentional NOESY spectra showed both microhelices to exhibit the same overall conformation at their 3'-CCA ends. Thermodynamic analysis and melting behaviour of the base-paired imino protons observed by NMR spectroscopy suggest that the A21G (A73G in tRNA) exchange results in a decrease of melting transition cooperativity and a destabilization of the terminal G1-C20 (G1-C72 in tRNA) base pair. Furthermore, the fact that this 3'-terminal imino proton is more solvent-exposed at physiological temperature might be another indication for the importance of the stability of the terminal base pair for specific tRNA recognition.
Starting Page	4551
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	22
Volume Number	25
Language	English
Publisher Date	1997-11-15
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in