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| Content Provider | PubMed Central |
|---|---|
| Author | Werber, M. M. Daphna, D. Goldman, J. Joseph, D. Radnay, J. Rozenszajn, L. A. |
| Abstract | Colony growth of T lymphocytes in a soft agar culture system, following stimulation with phytohaemagglutinin (PHA), is enhanced by T cell-derived conditioned medium (CM). The putative enhancing factor present in this CM has been termed lymphocyte colony-enhancing factor (LCEF). The purification of LCEF from CM has been complicated by the fact that active CM could be obtained only in the presence of 7-8 mg/ml of serum proteins. In order to circumvent this problem, CM which was shown to be active in the colony formation assay, was produced in the presence of a serum fraction precipitated by 1.6 M ammonium sulphate (AS). The first step of the purification of this CM was fractionation by 1.6 M AS, whereby the active material was fully recovered in the supernatant. The 20-fold purified material was applied on a DEAE-cellulose column. Two peaks of activity were obtained using the colony formation assay. The purification achieved by this second step was 5-10 fold for the first peak, eluting around 0.06 M NaCl, and two- to three-fold for the second peak, eluting around 0.19 M NaCl. Two peaks of activity were also obtained upon hydrophobic chromatography on a phenyl-Sepharose column, with enhancement factors of 5-8 for the first peak and 25-60 for the second peak. These results imply the presence of more than one LCEF molecule in the original CM. None of the LCEF-active fractions contained any T cell-growth factor (TCGF) activity; LCEF seems therefore to be a molecular entity distinct from TCGF. |
| Starting Page | 261 |
| File Format | |
| ISSN | 13652567 |
| e-ISSN | 13652567 |
| Journal | Immunology |
| Issue Number | 2 |
| Volume Number | 50 |
| Language | English |
| Publisher Date | 1983-10-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
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