NDLI: Chemotactic activity of human recombinant granulocyte-macrophage colony-stimulating factor.

Content Provider	PubMed Central
Author	Wang, J. M. Colella, S. Allavena, P. Mantovani, A.
Abstract	Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) seeded in the lower compartment of chemotaxis chambers induced migration of human peripheral blood monocytes and polymorphonuclear phagocytes. rhGM-CSF was active in inducing phagocyte migration at concentrations as low as 10 CML units/ml (corresponding to 0.002 microgram), and maximal activity was observed at 10(2)-10(4)/ml. Checkerboard analysis performed by seeding different concentrations of rhGM-CSF above and below the filter revealed that maximal induction of migration required a positive concentration gradient between the lower and upper compartment, and that rhGM-CSF elicited an actual chemotactic response in phagocytes. Human umbilical vein endothelial cells and blood large granular lymphocytes (LGL) responded poorly or not at all (endothelial cells) to rhGM-CSF under conditions in which appropriate reference chemoattractants were active. The chemotactic activity of rhGM-CSF may serve to recruit phagocytic cells from the blood compartment to amplify resistance against noxious agents.
Starting Page	439
File Format	PDF
ISSN	13652567
e-ISSN	13652567
Journal	Immunology
Issue Number	3
Volume Number	60
Language	English
Publisher Date	1987-03-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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Chemotactic activity of human recombinant granulocyte-macrophage colony-stimulating factor.