NDLI: The C-Terminal Half of Saccharomyces cerevisiae Mad1p Mediates Spindle Checkpoint Function, Chromosome Transmission Fidelity and CEN Association

Content Provider	PubMed Central
Author	Kastenmayer, James P. Lee, Marina S. Hong, Andrew L. Spencer, Forrest A. Basrai, Munira A.
Copyright Year	2005
Abstract	The evolutionarily conserved spindle checkpoint is a key mechanism ensuring high-fidelity chromosome transmission. The checkpoint monitors attachment between kinetochores and mitotic spindles and the tension between sister kinetochores. In the absence of proper attachment or tension, the spindle checkpoint mediates cell cycle arrest prior to anaphase. Saccharomyces cerevisiae Mad1p is required for the spindle checkpoint and for chromosome transmission fidelity. Moreover, Mad1p associates with the nuclear pore complex (NPC) and is enriched at kinetochores upon checkpoint activation. Using partial mad1 deletion alleles we determined that the C-terminal half of Mad1p is necessary and sufficient for checkpoint activation in response to microtubule depolymerizing agents, high-fidelity transmission of a reporter chromosome fragment, and in vivo association with centromeres, but not for robust NPC association. Thus, spindle checkpoint activation and chromosome transmission fidelity correlate and these Mad1p functions likely involve kinetochore association but not robust NPC association. These studies are the basis for elucidating the role of protein complexes containing Mad1p in the spindle checkpoint pathway and in maintaining genome stability in S. cerevisiae and other systems.
Related Links	http://dx.doi.org/10.1534/genetics.105.041426
Ending Page	517
Page Count	9
Starting Page	509
File Format	PDF
ISSN	00166731
Journal	Genetics
Issue Number	2
Volume Number	170
Language	English
Publisher	Genetics Society of America
Publisher Date	2005-04-01
Access Restriction	Open
Rights Holder	Genetics Society of America
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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