NDLI: Initiation factor-independent translation mediated by the hepatitis C virus internal ribosome entry site

Content Provider	PubMed Central
Author	Lancaster, Alissa M. Jan, Eric Peter, Sarnow
Copyright Year	2006
Abstract	The hepatitis C viral mRNA initiates translation using an internal ribosome entry site (IRES) located in the 5′ noncoding region of the viral genome. At physiological magnesium ion concentrations, the HCV IRES forms a binary complex with the 40S ribosomal subunit, recruits initiation factor eIF3 and the ternary eIF2/GTP/Met-tRNAi Met complex, and joins 60S subunits to assemble translation-competent 80S ribosomes. Here we show that in the presence of 5 mM MgCl2, the HCV IRES can initiate translation by an alternative mechanism that does not require known initiation factors. Specifically, the HCV IRES was shown to initiate translation in a reconstituted system consisting only of purified 40S and 60S subunits, elongation factors, and aminoacylated tRNAs at high magnesium concentration. Analyses of assembled complexes supported a mechanism by which preformed 80S ribosomes can assemble directly on the HCV IRES at high cation concentrations. This mechanism is reminiscent of that employed by the divergent IRES elements in the Dicistroviridae, exemplified by the cricket paralysis virus, which mediates initiation of protein synthesis without initiator tRNA.
Related Links	http://dx.doi.org/10.1261/rna.2342306
Starting Page	894
File Format	PDF
ISSN	14699001
e-ISSN	14699001
Journal	RNA
Issue Number	5
Volume Number	12
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2006-05-01
Access Restriction	Open
Rights Holder	Cold Spring Harbor Laboratory Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology

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