NDLI: Pneumococcal Polysaccharides Interact with Human Dendritic Cells

Content Provider	PubMed Central
Author	Meltzer, Ulrike Goldblatt, David
Copyright Year	2006
Abstract	Dendritic cells (DCs) are critical antigen presentation cells whose influence on murine immune responses to polysaccharide antigens has only recently been elucidated. Little is known about human DC-polysaccharide interactions. We set out to study the interaction between human monocyte-derived DCs and pneumococcal capsular polysaccharides (PPS) in vitro. Immature DCs were generated from peripheral blood monocytes and incubated with fluorescein isothiocyanate-labeled PPS type 9N or 14 for assessment of uptake. DCs were exposed to PPS type 1, 6B, 9N, 14, 19F, or 23F in the absence or presence of Escherichia coli lipopolysaccharide (LPS) for assessment of phenotypic DC maturation and cytokine production. PPS were taken up by immature DCs and proceeded to HLA-DR+ and lysosome-associated membrane protein-1+ late endosomal compartments. Uptake was reduced in the presence of cytochalasin D and wortmannin, suggesting that both cytoskeletal rearrangements and phosphatidylinositol 3-kinase activation may be required for internalization. None of the PPS tested induced DC phenotype changes, maturation, or interleukin-12 (IL-12)/IL-10 production. However, PPS were capable of modulating the response of the DCs to a second signal such as LPS. Exposure of DCs to PPS in the presence of LPS resulted in an altered cytokine balance with significantly increased IL-10 production and reduced IL-12 production compared to LPS alone. This effect was not seen using the control antigen tetanus toxoid. DC-pneumococcus interaction may affect subsequent immune responses to pneumococci, as an altered cytokine balance may have a profound effect on DC-driven T-cell priming.
Related Links	http://dx.doi.org/10.1128/iai.74.3.1890-1895.2006
Ending Page	1895
Page Count	6
Starting Page	1890
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	3
Volume Number	74
Language	English
Publisher	American Society for Microbiology
Publisher Date	2006-03-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Pneumococcal Conjugate Vaccines Overcome Splenic Dependency of Antibody Response to Pneumococcal Polysaccharides

Pneumococcal polysaccharides complexed with C3d bind to human B lymphocytes via complement receptor type 2.

Inhibition of CD1 Expression in Human Dendritic Cells during Intracellular Infection with Leishmania donovani

Interaction of Pneumococcal Histidine Triad Proteins with Human Complement▿

Pneumococcal adherence to human epithelial cells.

Interaction of Neisseria meningitidis with Human Dendritic Cells

Antigenicity of type-specific pneumococcal polysaccharides in rats.

Filarial Antigens Impair the Function of Human Dendritic Cells during Differentiation

Interactions of Salmonella enterica with dendritic cells

Pneumococcal Polysaccharides Interact with Human Dendritic Cells

Similar Documents

Pneumococcal Conjugate Vaccines Overcome Splenic Dependency of Antibody Response to Pneumococcal Polysaccharides

Pneumococcal polysaccharides complexed with C3d bind to human B lymphocytes via complement receptor type 2.

Inhibition of CD1 Expression in Human Dendritic Cells during Intracellular Infection with Leishmania donovani

Interaction of Pneumococcal Histidine Triad Proteins with Human Complement▿

Pneumococcal adherence to human epithelial cells.

Interaction of Neisseria meningitidis with Human Dendritic Cells

Antigenicity of type-specific pneumococcal polysaccharides in rats.

Filarial Antigens Impair the Function of Human Dendritic Cells during Differentiation

Interactions of Salmonella enterica with dendritic cells

Pneumococcal Polysaccharides Interact with Human Dendritic Cells