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| Content Provider | PubMed Central |
|---|---|
| Author | Mcneil, Elizabeth Capaldo, Christopher T. Macara, Ian G. |
| Editor | Asma, Nusrat |
| Copyright Year | 2006 |
| Abstract | Zonula occludens (ZO)-1 was the first tight junction protein to be cloned and has been implicated as an important scaffold protein. It contains multiple domains that bind a diverse set of junction proteins. However, the molecular functions of ZO-1 and related proteins such as ZO-2 and ZO-3 have remained unclear. We now show that gene silencing of ZO-1 causes a delay of ∼3 h in tight junction formation in Madin-Darby canine kidney (MDCK) epithelial cells, but mature junctions seem functionally normal even in the continuing absence of ZO-1. Depletion of ZO-2, cingulin, or occludin, proteins that can interact with ZO-1, had no discernible effects on tight junctions. Rescue of junction assembly using murine ZO-1 mutants demonstrated that the ZO-1 C terminus is neither necessary nor sufficient for normal assembly. Moreover, mutation of the PDZ1 domain did not block rescue. However, point mutations in the Src homology 3 (SH3) domain almost completely prevented rescue. Surprisingly, the isolated SH3 domain of ZO-1 could also rescue junction assembly. These data reveal an unexpected function for the SH3 domain of ZO-1 in regulating tight junction assembly in epithelial cells and show that cingulin, occludin, or ZO-2 are not limiting for junction assembly in MDCK monolayers. |
| Related Links | http://dx.doi.org/10.1091/mbc.e05-07-0650 |
| Ending Page | 1932 |
| Page Count | 11 |
| Starting Page | 1922 |
| File Format | |
| ISSN | 10591524 |
| Journal | Molecular Biology of the Cell |
| Issue Number | 4 |
| Volume Number | 17 |
| Language | English |
| Publisher | The American Society for Cell Biology |
| Publisher Date | 2006-01-01 |
| Access Restriction | Open |
| Rights Holder | The American Society for Cell Biology |
| Subject Keyword | Cell Biology Molecular Biology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology |
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