NDLI: The fusion domain of HIV gp41 interacts specifically with heparan sulfate on the T-lymphocyte cell surface

Content Provider	PubMed Central
Author	Cladera, Josep Martin, Isabelle O’shea, Paul
Copyright Year	2001
Abstract	Studies of the interaction of the 16 residue fusion peptide domain of human immunodeficiency virus glycoprotein gp41 (gp41FD) with T lymphocytes are outlined. Fluorescence measurements of changes in the electrostatic surface and dipole potentials of the plasma membrane following the interaction with gp41FD are described. The results show that gp41FD interacts with heparan sulfate located on the cell surface. This interaction is blocked by interleukin-8 and abolished by pre-treating the cells with heparitinase. The specificity of the reaction was also assessed by observations that soluble heparan sulfate competes with the cell membrane interaction whereas soluble heparin (at the levels utilized) does not. Following binding to heparan sulfate, the interaction with the membrane seems to take place in a cooperative manner with the formation of gp41FD trimers. In simpler phospholipid membranes, however, a trimeric complex does not appear to be the dominant mode of interaction. Finally, by repeating some of these studies within an imaging regime, it appears that the gp41FD–T-cell interaction takes place within specific domains on the cell surface to similarly localized heparan sulfate moieties.
Related Links	http://dx.doi.org/10.1093/emboj/20.1.19
Ending Page	26
Page Count	8
Starting Page	19
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	1-2
Volume Number	20
Language	English
Publisher	Oxford University Press
Publisher Date	2001-01-15
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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