NDLI: Helicobacter pylori interstrain restriction-modification diversity prevents genome subversion by chromosomal DNA from competing strains

Content Provider	PubMed Central
Author	Aras, Rahul A. Small, Aaron J. Ando, Takafumi Blaser, Martin J.
Copyright Year	2002
Abstract	Helicobacter pylori, bacteria that colonize the human gastric mucosa, possess a large number of genes for restriction-modification (R-M) systems, and essentially, every strain possesses a unique complement of functional and partial R-M systems. Nearly half of the H.pylori strains studied possess an active type IIs R-M system, HpyII, with the recognition sequence GAAGA. Recombination between direct repeats that flank the R-M cassette allows for its deletion whereas strains lacking hpyIIRM can acquire this cassette through natural transformation. We asked whether strains lacking HpyII R-M activity can acquire an active hpyIIRM cassette [containing a 1.4 kb kanamycin resistance (aphA) marker], whether such acquisition is DNase sensitive or resistant and whether restriction barriers limit acquisition of chromosomal DNA. Our results indicate that natural transformation and conjugation-like mechanisms may contribute to the transfer of large (4.8 kb) insertions of chromosomal DNA between H.pylori strains, that inactive or partial R-M systems can be reactivated upon recombination with a functional allele, consistent with their being contingency genes, and that H.pylori R-M diversity limits acquisition of chromosomal DNA fragments of ≥1 kb.
Starting Page	5391
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	24
Volume Number	30
Language	English
Publisher	Oxford University Press
Publisher Date	2002-12-15
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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Helicobacter pylori interstrain restriction‐modification diversity prevents genome subversion by chromosomal DNA from competing strains

Comparative genomics of the restriction-modification systems in Helicobacter pylori.

Identification of type II restriction and modification systems in Helicobacter pylori reveals their substantial diversity among strains

Functional analysis of putative restriction–modification system genes in the Helicobacter pylori J99 genome

Characterization of chromosomal DNA profiles from Helicobacter pylori strains isolated from sequential gastric biopsy specimens.

The Helicobacter pylori HpyAXII restriction–modification system limits exogenous DNA uptake by targeting GTAC sites but shows asymmetric conservation of the DNA methyltransferase and restriction endonuclease components

Identification of type II restriction and modification systems in Helicobacter pylori reveals their substantial diversity among strains.

Functional analysis of putative restriction–modification system genes in the Helicobacter pylori J99 genome

A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains.

Helicobacter pylori interstrain restriction-modification diversity prevents genome subversion by chromosomal DNA from competing strains

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Helicobacter pylori interstrain restriction‐modification diversity prevents genome subversion by chromosomal DNA from competing strains

Comparative genomics of the restriction-modification systems in Helicobacter pylori.

Identification of type II restriction and modification systems in Helicobacter pylori reveals their substantial diversity among strains

Functional analysis of putative restriction–modification system genes in the Helicobacter pylori J99 genome

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The Helicobacter pylori HpyAXII restriction–modification system limits exogenous DNA uptake by targeting GTAC sites but shows asymmetric conservation of the DNA methyltransferase and restriction endonuclease components

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