NDLI: Meeting Report: Application of Genotyping Methods to Assess Risks from Cryptosporidium in Watersheds

Content Provider	PubMed Central
Author	Ferguson, Christobel Deere, Dan Sinclair, Martha Chalmers, Rachel M. Elwin, Kristin Hadfield, Stephen Xiao, Lihua Ryan, Una Gasser, Robin Abs, El-osta Youssef Stevens, Melita
Copyright Year	2006
Abstract	A workshop titled “Application of Genotyping Methods to Assess Pathogen Risks from Cryptosporidium in Drinking Water Catchments” was held at the International Water Association biennial conference, Marrakech, Morocco, 23 September 2004. The workshop presented and discussed the findings of an interlaboratory trial that compared methods for genotyping Cryptosporidium oocysts isolated from feces. The primary goal of the trial and workshop was to assess the utility of current Cryptosporidium genotyping methods for determining the public health significance of oocysts isolated from feces in potable-water–supply watersheds. An expert panel of 16 watershed managers, public health practitioners, and molecular parasitologists was assembled for the workshop. A subordinate goal of the workshop was to educate watershed management and public health practitioners. An open invitation was extended to all conference delegates to attend the workshop, which drew approximately 50 interested delegates. In this report we summarize the peer consensus emerging from the workshop. Recommendations on the use of current methods by watershed managers and public health practitioners were proposed. Importantly, all the methods that were reported in the trial were mutually supporting and found to be valuable and worthy of further utility and development. Where there were choices as to which method to apply, the small-subunit ribosomal RNA gene was considered to be the optimum genetic locus to target. The single-strand conformational polymorphism method was considered potentially the most valuable for discriminating to the subtype level and where a large number of samples were to be analyzed. A research agenda for protozoan geneticists was proposed to improve the utility of methods into the future. Standardization of methods and nomenclature was promoted.
Related Links	http://dx.doi.org/10.1289/ehp.8240
Ending Page	434
Page Count	5
Starting Page	430
File Format	PDF
ISSN	00916765
e-ISSN	15529924
Journal	Environmental Health Perspectives
Issue Number	3
Volume Number	114
Language	English
Publisher	National Institute of Environmental Health Sciences
Publisher Date	2005-10-01
Access Restriction	Open
Rights Holder	National Institute of Environmental Health Sciences
Subject Keyword	Public Health, Environmental and Occupational Health Health, Toxicology and Mutagenesis Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health

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