NDLI: Factors Affecting the Determination of Respiratory Activity on the Basis of Cyanoditolyl Tetrazolium Chloride Reduction with Membrane Filtration

Please wait, while we are loading the content...

Year: 2016, Volume: 82

Year: 2015, Volume: 82

Year: 2015, Volume: 81

Year: 2014, Volume: 80

Year: 2013, Volume: 79

Year: 2012, Volume: 78

Year: 2011, Volume: 77

Year: 2010, Volume: 76

Year: 2009, Volume: 75

Year: 2008, Volume: 74

Year: 2007, Volume: 73

Year: 2006, Volume: 72

Year: 2005, Volume: 71

Year: 2004, Volume: 70

Year: 2003, Volume: 69

Year: 2002, Volume: 69

Year: 2002, Volume: 68

Year: 2001, Volume: 67

Year: 2000, Volume: 66

Year: 1999, Volume: 65

Year: 1998, Volume: 64

Year: 1997, Volume: 63

Year: 1996, Volume: 62

Year: 1995, Volume: 61

Year: 1995, Volume: 61, Issue: 12

A direct PCR detection method for Clostridium tyrobutyricum spores in up to 100 milliliters of raw milk.

Entry of Escherichia coli into stationary phase is indicated by endogenous and exogenous accumulation of nucleobases.

Uptake of Benzoic Acid and Chloro-Substituted Benzoic Acids by Alcaligenes denitrificans BRI 3010 and BRI 6011

Characterization of Serratia entomophila Bacteriophages and the Phage-Resistant Mutant Strain BC4B

Polymer production by Klebsiella pneumoniae 4-hydroxyphenylacetic acid hydroxylase genes cloned in Escherichia coli.

Death of enterohemorrhagic Escherichia coli O157:H7 in real mayonnaise and reduced-calorie mayonnaise dressing as influenced by initial population and storage temperature.

Modification of kanamycin-esculin-azide agar to improve selectivity in the enumeration of fecal streptococci from water samples.

Xylose-metabolizing Saccharomyces cerevisiae strains overexpressing the TKL1 and TAL1 genes encoding the pentose phosphate pathway enzymes transketolase and transaldolase.

Utilization of Halogenated Benzenes, Phenols, and Benzoates by Rhodococcus opacus GM-14

Survival of, and induced stress resistance in, carbon-starved Pseudomonas fluorescens cells residing in soil.

Bioconversion of 2,4-diamino-6-nitrotoluene to a novel metabolite under anoxic and aerobic conditions.

UV B-Induced Vertical Migrations of Cyanobacteria in a Microbial Mat

Structure and function of Tn5467, a Tn21-like transposon located on the Thiobacillus ferrooxidans broad-host-range plasmid pTF-FC2.

Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.

Comparison of Biomass Dry Weights and Radial Growth Rates of Fungal Colonies on Media Solidified with Different Gelling Compounds

Effects of a Bacterial Hay Preservative (Pediococcus pentosaceus) on Hay under Experimental Storage Conditions

A general method for the consecutive integration of single copies of a heterologous gene at multiple locations in the Bacillus subtilis chromosome by replacement recombination.

Yeast succession in the Amazon fruit Parahancornia amapa as resource partitioning among Drosophila spp.

Organic Solvent-Tolerant Bacterium Which Secretes an Organic Solvent-Stable Proteolytic Enzyme

Genetic diversity of Burkholderia solanacearum (synonym Pseudomonas solanacearum) race 3 in Kenya.

Rapid differentiation of bacterial species with multiple probes of different lengths in a single slot blot hybridization.

Demonstration of Laccase in the White Rot Basidiomycete Phanerochaete chrysosporium BKM-F1767

Differential Inhibition by Allylsulfide of Nitrification and Methane Oxidation in Freshwater Sediment

Cloning and characterization of the genes for p-nitrobenzoate degradation from Pseudomonas pickettii YH105.

Effect of point-of-use, activated carbon filters on the bacteriological quality of rural groundwater supplies.

Direct detection of recombinant gene expression by two genetically engineered yeasts in soil on the transcriptional and translational levels.

Factors Affecting the Determination of Respiratory Activity on the Basis of Cyanoditolyl Tetrazolium Chloride Reduction with Membrane Filtration

Differentiation of epidemic-associated strains of Listeria monocytogenes by restriction fragment length polymorphism in a gene region essential for growth at low temperatures (4 degrees C).

Cloning and DNA sequence analysis of two abortive infection phage resistance determinants from the lactococcal plasmid pNP40.

Identification of two pigments and a hydroxystilbene antibiotic from Photorhabdus luminescens.

Triggering Glutamate Excretion in Corynebacterium glutamicum by Modulating the Membrane State with Local Anesthetics and Osmotic Gradients

Isolation of Multiple Subspecies of Bacillus thuringiensis from a Population of the European Sunflower Moth, Homoeosoma nebulella

Sequencing and analysis of the prolate-headed lactococcal bacteriophage c2 genome and identification of the structural genes.

Direct and Rapid Analysis of the Adhesion of Bacteria to Solid Surfaces: Interaction of Fluorescently Labeled Rhodococcus Strain GIN-1 (NCIMB 40340) Cells with Titanium-Rich Particles

Sulfur-Specific Microbial Desulfurization of Sterically Hindered Analogs of Dibenzothiophene

Comparative Serology of the Marine Fish Pathogen Vibrio anguillarum

Phenolic Azo Dye Oxidation by Laccase from Pyricularia oryzae

Role of Glycine Betaine and Related Osmolytes in Osmotic Stress Adaptation in Yersinia enterocolitica ATCC 9610

Halotolerance in Methanosarcina spp.: Role of N(sup(epsilon))-Acetyl-(beta)-Lysine, (alpha)-Glutamate, Glycine Betaine, and K(sup+) as Compatible Solutes for Osmotic Adaptation

Statistical approach for comparison of the growth rates of five strains of Staphylococcus aureus.

Degradation and utilization of xylan by the ruminal bacteria Butyrivibrio fibrisolvens and Selenomonas ruminantium.

Cloning, sequencing, and expression of a xylanase gene from the extreme thermophile Dictyoglomus thermophilum Rt46B.1 and activity of the enzyme on fiber-bound substrate.

Demonstration of Aflatoxin Inhibitory Activity in a Cotton Seed Coat Xylan

Isolation and Characterization of Clostridium butyricum DSM 5431 Mutants with Increased Resistance to 1,3-Propanediol and Altered Production of Acids

Detection of small round structured viruses in shellfish by reverse transcription-PCR.

Comparison of the Growth and Survival of Larval Turbot in the Absence of Culturable Bacteria with Those in the Presence of Vibrio anguillarum, Vibrio alginolyticus, or a Marine Aeromonas sp

Aflavinines and other antiinsectan metabolites from the ascostromata of Eupenicillium crustaceum and related species.

Natural assemblages of marine bacteria exhibiting high-speed motility and large accelerations.

Genome analysis of Clostridium botulinum type A by pulsed-field gel electrophoresis.

In situ 31P nuclear magnetic resonance for observation of polyphosphate and catabolite responses of chemostat-cultivated Saccharomyces cerevisiae after alkalinization.

Purification and characterization of an extracellular beta-1,4-mannanase from a marine bacterium, Vibrio sp. strain MA-138.

Purification and partial amino acid sequence of plantaricin S, a bacteriocin produced by Lactobacillus plantarum LPCO10, the activity of which depends on the complementary action of two peptides.

Gentamicin-Containing Peptone-Yeast Extract Medium for Cocultivation of Hartmannella vermiformis ATCC 50256 and Virulent Strains of Legionella pneumophila

Purification and properties of xylanase A from alkali-tolerant Bacillus sp. strain BP-23.

Bacillus subtilis Vegetative Catalase Is an Extracellular Enzyme

Mutations in the trpD gene of Corynebacterium glutamicum confer 5-methyltryptophan resistance by encoding a feedback-resistant anthranilate phosphoribosyltransferase.

Population Sizes and Growth Pressure Responses of Intestinal Microfloras of Deep-Sea Fish Retrieved from the Abyssal Zone

Cloning, sequencing, and expression in Escherichia coli of the Bacillus pumilus gene for ferulic acid decarboxylase.

Distribution and properties of fructosyl amino acid oxidase in fungi.

Alkalophilic Bacillus sp. strain LG12 has a series of serine protease genes.

Arginine deiminase system and acid adaptation of oral streptococci.

Cellular Fatty Acid Profiles of Lactobacillus and Lactococcus Strains in Relation to the Oleic Acid Content of the Cultivation Medium

Substrate diversity and expression of the 2,4,5-trichlorophenoxyacetic acid oxygenase from Burkholderia cepacia AC1100.

Development of a sensitive chemiluminometric assay for the detection of beta-galactosidase in permeabilized coliform bacteria and comparison with fluorometry and colorimetry.

Multiple Polychlorinated Biphenyl Transformation Systems in the Gram-Positive Bacterium Rhodococcus sp. Strain RHA1

Detection of viable Cryptosporidium parvum oocysts by PCR.

Phylogenetic diversity of subsurface marine microbial communities from the Atlantic and Pacific oceans.

Year: 1995, Volume: 61, Issue: 11

Year: 1995, Volume: 61, Issue: 10

Year: 1995, Volume: 61, Issue: 9

Year: 1995, Volume: 61, Issue: 8

Year: 1995, Volume: 61, Issue: 7

Year: 1995, Volume: 61, Issue: 6

Year: 1995, Volume: 61, Issue: 5

Year: 1995, Volume: 61, Issue: 4

Year: 1995, Volume: 61, Issue: 3

Year: 1995, Volume: 61, Issue: 2

Year: 1995, Volume: 61, Issue: 1

Year: 1994, Volume: 60

Year: 1993, Volume: 59

Year: 1992, Volume: 58

Year: 1991, Volume: 57

Year: 1990, Volume: 56

Year: 1989, Volume: 55

Year: 1988, Volume: 54

Year: 1987, Volume: 53

Year: 1986, Volume: 52

Year: 1986, Volume: 51

Year: 1985, Volume: 50

Year: 1985, Volume: 49

Year: 1984, Volume: 48

Year: 1984, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1981, Volume: 42

Year: 1981, Volume: 41

Year: 1980, Volume: 40

Year: 1980, Volume: 39

Year: 1979, Volume: 38

Year: 1979, Volume: 37

Year: 1978, Volume: 36

Year: 1978, Volume: 35

Year: 1977, Volume: 34

Year: 1977, Volume: 33

Year: 1976, Volume: 32

Year: 1976, Volume: 31

Factors Affecting the Determination of Respiratory Activity on the Basis of Cyanoditolyl Tetrazolium Chloride Reduction with Membrane Filtration

Content Provider	PubMed Central
Author	Pyle, B. H. Broadaway, S. C. Mcfeters, G. A.
Abstract	Deficiencies in traditional bacterial enumeration techniques which rely on colony formation have led to the use of total direct counting methods, such as the acridine orange direct count technique for the enumeration of planktonic bacteria. As total direct counts provide no information on the viability or activity of the organisms, demonstration of respiratory activity with the fluorochrome cyanoditolyl tetrazolium chloride (CTC) has been employed. We have modified this technique by performing filtration prior to CTC incubation. Cells captured on a polycarbonate membrane were incubated on absorbent pads saturated with medium containing CTC. Following counterstaining with DAPI (4(prm1),6-diamidino-2-phenylindole) total and respiring cells were enumerated by epifluorescence microscopy. Factors affecting CTC reduction by Klebsiella pneumoniae, Salmonella typhimurium, and Escherichia coli K-12 were investigated. With K. pneumoniae, nutrient additions to the CTC medium did not increase the number of respiring cells detected. CTC reduction by all three organisms decreased in response to an increase of the pH of the CTC medium above pH 6.5. Increasing phosphate concentrations contributed to this inhibitory effect. CTC-membrane filter counts of K. pneumoniae, S. typhimurium, and E. coli K-12 and of bacteria in well water corresponded closely with plate counts (r = 0.987). The results show that careful attention should be given to the composition of CTC-containing media which are used to enumerate respiring bacteria. With an appropriate medium, reliable enumeration of respiring bacteria can be achieved within a few hours.
Starting Page	4304
File Format	PDF
ISSN	10985336
e-ISSN	10985336
Journal	Applied and Environmental Microbiology
Issue Number	12
Volume Number	61
Language	English
Publisher Date	1995-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Ecology Food Science Applied Microbiology and Biotechnology Biotechnology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in