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| Content Provider | PubMed Central |
|---|---|
| Author | Wong, J. Y. Opdebeeck, J. P. |
| Abstract | Triton X-100, SDS, n-octyl glucoside, and conditions of alkaline pH and high and low ionic strength, were used to solubilize membranes prepared from the mid-gut of the cattle tick Boophilus microplus. Hereford cattle (Bos taurus) were immunized with soluble membrane antigens in saponin, and then challenged twice with 20,000 tick larvae, 7 days apart. All soluble extracts, except that extracted with high ionic strength buffer, provided significant protection (56-78%) (P < 0.05) to immunized cattle compared with control cattle. Precipitation of the soluble n-octyl glucoside extract did not alter the immunogenicity of this vaccine. Where poor responses to membrane vaccines were obtained (< 60% protection), booster injections of antigen effected increased protection. The experimental cattle were typed for bovine major histocompatibility system class 1 antigens. The presence of three antigens (MB5, MB14 and MB20) had a significant effect on protective immunity induced by the membrane vaccines (P < 0.05). Protein profiles of Triton X-100, n-octyl glucoside and low ionic strength buffer extracts were compared by high-performance size-exclusion chromatography. Tick membranes extracted by either non-ionic detergents or low ionic strength solutions provided the best source of protective antigens. |
| Starting Page | 149 |
| File Format | |
| ISSN | 13652567 |
| e-ISSN | 13652567 |
| Journal | Immunology |
| Issue Number | 1 |
| Volume Number | 66 |
| Language | English |
| Publisher Date | 1989-01-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
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