NDLI: Protection against tuberculosis by bone marrow cells expressing mycobacterial hsp65.

Content Provider	PubMed Central
Author	Silva, C. L. Pietro, R. L. Januario, A. Silva, M. F. Da Lowrie, D. B. Bonato, V. L. Lima, V. M.
Abstract	Although mice acquire only a slight degree of protection against tuberculosis by immunization with Mycobacterium leprae (M. leprae) hsp65 in incomplete Freund's adjuvant, protection is substantial following immunization by injection with J774 macrophage-like tumour cells that express the protein from the mycobacterial gene via a retroviral vector. We here took the same vector, used it to transfect the gene into normal murine bone marrow cells in vitro, and then used the transfected cells to reconstitute haematopoiesis in lethally irradiated mice. Bone marrow-cell clonal expansion and production of the protein in vivo resulted in specific delayed-type hypersensitivity and protection against challenge with Mycobacterium tuberculosis (M. tuberculosis) in about half of recipients. Counts of live bacteria in liver at 3 weeks were fivefold lower in delayed-type hypersensitivity (DTH)-positive than in DTH-negative mice. Other mice acquired neither DTH nor protection despite the presence of the protein in peripheral blood.
Starting Page	519
File Format	PDF
ISSN	13652567
e-ISSN	13652567
Journal	Immunology
Issue Number	4
Volume Number	86
Language	English
Publisher Date	1995-12-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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Protection against tuberculosis by bone marrow cells expressing mycobacterial hsp65.