NDLI: Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Sedimentation Coefficient Distributions c(s) and Asymptotic Boundary Profiles from Gilbert-Jenkins Theory

Content Provider	PubMed Central
Author	Dam, Julie Schuck, Peter
Copyright Year	2005
Abstract	Interacting proteins in rapid association equilibrium exhibit coupled migration under the influence of an external force. In sedimentation, two-component systems can exhibit bimodal boundaries, consisting of the undisturbed sedimentation of a fraction of the population of one component, and the coupled sedimentation of a mixture of both free and complex species in the reaction boundary. For the theoretical limit of diffusion-free sedimentation after infinite time, the shapes of the reaction boundaries and the sedimentation velocity gradients have been predicted by Gilbert and Jenkins. We compare these asymptotic gradients with sedimentation coefficient distributions, c(s), extracted from experimental sedimentation profiles by direct modeling with superpositions of Lamm equation solutions. The overall shapes are qualitatively consistent and the amplitudes and weight-average s-values of the different boundary components are quantitatively in good agreement. We propose that the concentration dependence of the area and weight-average s-value of the c(s) peaks can be modeled by isotherms based on Gilbert-Jenkins theory, providing a robust approach to exploit the bimodal structure of the reaction boundary for the analysis of experimental data. This can significantly improve the estimates for the determination of binding constants and hydrodynamic parameters of the complexes.
Related Links	http://dx.doi.org/10.1529/biophysj.105.059584
Starting Page	651
File Format	PDF
ISSN	15420086
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	1
Volume Number	89
Language	English
Publisher	Biophysical Society
Publisher Date	2005-07-01
Access Restriction	Open
Rights Holder	Biophysical Society
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Lamm Equation Modeling and Sedimentation Coefficient Distributions c(s)

On computational approaches for size-and-shape distributions from sedimentation velocity analytical ultracentrifugation

Simulation of Gilbert Theory for Self-Association in Sedimentation Velocity Experiments: A Guide to Evaluate Best Fitting Models

Macromolecular Size-and-Shape Distributions by Sedimentation Velocity Analytical Ultracentrifugation

Diffusion of the Reaction Boundary of Rapidly Interacting Macromolecules in Sedimentation Velocity

Molecular mass determination by sedimentation velocity experiments and direct fitting of the concentration profiles.

On the analysis of sedimentation velocity in the study of protein complexes

Advances in sedimentation velocity analysis.

Analytical ultracentrifugation as a tool for studying protein interactions

Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Sedimentation Coefficient Distributions c(s) and Asymptotic Boundary Profiles from Gilbert-Jenkins Theory

Similar Documents

Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Lamm Equation Modeling and Sedimentation Coefficient Distributions c(s)

On computational approaches for size-and-shape distributions from sedimentation velocity analytical ultracentrifugation

Simulation of Gilbert Theory for Self-Association in Sedimentation Velocity Experiments: A Guide to Evaluate Best Fitting Models

Macromolecular Size-and-Shape Distributions by Sedimentation Velocity Analytical Ultracentrifugation

Diffusion of the Reaction Boundary of Rapidly Interacting Macromolecules in Sedimentation Velocity

Molecular mass determination by sedimentation velocity experiments and direct fitting of the concentration profiles.

On the analysis of sedimentation velocity in the study of protein complexes

Advances in sedimentation velocity analysis.

Analytical ultracentrifugation as a tool for studying protein interactions

Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Sedimentation Coefficient Distributions c(s) and Asymptotic Boundary Profiles from Gilbert-Jenkins Theory