NDLI: Identification and Localization of Flagellins FlaA and FlaB3 within Flagella of Methanococcus voltae

Content Provider	PubMed Central
Author	Bardy, Sonia L. Mori, Takahisa Kaoru, Komoriya Aizawa, Shin-ichi Jarrell, Ken F.
Copyright Year	2002
Abstract	Methanococcus voltae possesses four flagellin genes, two of which (flaB1 and flaB2) have previously been reported to encode major components of the flagellar filament. The remaining two flagellin genes, flaA and flaB3, are transcribed at lower levels, and the corresponding proteins remained undetected prior to this work. Electron microscopy examination of flagella isolated by detergent extraction of whole cells revealed a curved, hook-like region of varying length at the end of a long filament. Enrichment of the curved region of the flagella resulted in the identification of FlaB3 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and N-terminal sequencing, and the localization of this flagellin to the cell-proximal portion of the flagellum was confirmed through immunoblotting and immunoelectron microscopy with FlaB3-specific antibodies, indicating that FlaB3 likely composes the curved portion of the flagella. This could represent a unique case of a flagellin performing the role of the bacterial hook protein. FlaA-specific antibodies were used in immunoblotting to determine that FlaA is found throughout the flagellar filament. M. voltae cells were transformed with a modified flaA gene containing a hemagglutinin (HA) tag introduced into the variable region. Transformants that had replaced the wild-type copy of the flaA gene with the HA-tagged version incorporated the HA-tagged version of FlaA into flagella which appeared normal by electron microscopy.
Related Links	http://dx.doi.org/10.1128/jb.184.19.5223-5233.2002
Ending Page	5233
Page Count	11
Starting Page	5223
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	19
Volume Number	184
Language	English
Publisher	American Society for Microbiology
Publisher Date	2002-10-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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Cloning and sequencing of a multigene family encoding the flagellins of Methanococcus voltae.

Isolation of flagella from the archaebacterium Methanococcus voltae by phase separation with Triton X-114.

Identification of the Methanococcus voltae S-layer structural gene.

Identification of a vanadate-sensitive, membrane-bound ATPase in the archaebacterium Methanococcus voltae.

Nutrition and carbon metabolism of Methanococcus voltae.

Chemotaxis in the archaebacterium Methanococcus voltae.

Identification and Characterization of the Unique N-Linked Glycan Common to the Flagellins and S-layer Glycoprotein of Methanococcus voltae

Posttranslational Processing of Methanococcus voltae Preflagellin by Preflagellin Peptidases of M. voltae and Other Methanogens

Heat shock response of the archaebacterium Methanococcus voltae.

Identification and Localization of Flagellins FlaA and FlaB3 within Flagella of Methanococcus voltae

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Cloning and sequencing of a multigene family encoding the flagellins of Methanococcus voltae.

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Identification and Localization of Flagellins FlaA and FlaB3 within Flagella of Methanococcus voltae