NDLI: Two Regions of GerE Required for Promoter Activation in Bacillus subtilis

Content Provider	PubMed Central
Author	Crater, Dinene L. Moran, Charles P.
Copyright Year	2002
Abstract	GerE from Bacillus subtilis is the smallest member of the LuxR-FixJ family of transcription activators. Its 74-amino-acid sequence is similar over its entire length to the DNA binding domain of this protein family, including a putative helix-turn-helix (HTH) motif. In this report, we sought to define regions of GerE involved in promoter activation. We examined the effects of single alanine substitutions at 19 positions that were predicted by the crystal structure of GerE to be located on its surface. A single substitution of alanine for the phenylalanine at position 6 of GerE (F6A) resulted in decreased transcription in vivo and in vitro from the GerE-dependent cotC promoter. However, the F6A substitution had little effect on transcription from the GerE-dependent cotX promoter. In contrast, a single alanine substitution for the leucine at position 67 (L67A) reduced transcription from the cotX promoter, but not from the cotC promoter. The results of DNase I protection assays and in vitro transcription reactions lead us to suggest that the F6A and L67A substitutions define two regions of GerE, activation region 1 (AR1) and AR2, that are required for activation of the cotC and cotX promoters, respectively. A comparison of our results with those from studies of MalT and BvgA indicated that other members of the LuxR-FixJ family may use more than one surface to interact with RNA polymerase during promoter activation.
Related Links	http://dx.doi.org/10.1128/jb.184.1.241-249.2002
Ending Page	249
Page Count	9
Starting Page	241
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	1
Volume Number	184
Language	English
Publisher	American Society for Microbiology
Publisher Date	2002-01-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A Region of ςK Involved in Promoter Activation by GerE in Bacillus subtilis

α-Helix E of Spo0A Is Required for σA- but Not for σH-Dependent Promoter Activation in Bacillus subtilis

Identification of a DNA Binding Region in GerE from Bacillus subtilis

A Region in Bacillus subtilis ςH Required for Spo0A-Dependent Promoter Activity

Two-component regulatory proteins ResD-ResE are required for transcriptional activation of fnr upon oxygen limitation in Bacillus subtilis.

Cloning and characterization of DNA damage-inducible promoter regions from Bacillus subtilis.

An additional GerE-controlled gene encoding an abundant spore coat protein from Bacillus subtilis.

Spo0A-Dependent Activation of an Extended −10 Region Promoter in Bacillus subtilis

Mutational Analysis of the phoD Promoter in Bacillus subtilis: Implications for PhoP Binding and Promoter Activation of Pho Regulon Promoters

Two Regions of GerE Required for Promoter Activation in Bacillus subtilis

Similar Documents

A Region of ςK Involved in Promoter Activation by GerE in Bacillus subtilis

α-Helix E of Spo0A Is Required for σA- but Not for σH-Dependent Promoter Activation in Bacillus subtilis

Identification of a DNA Binding Region in GerE from Bacillus subtilis

A Region in Bacillus subtilis ςH Required for Spo0A-Dependent Promoter Activity

Two-component regulatory proteins ResD-ResE are required for transcriptional activation of fnr upon oxygen limitation in Bacillus subtilis.

Cloning and characterization of DNA damage-inducible promoter regions from Bacillus subtilis.

An additional GerE-controlled gene encoding an abundant spore coat protein from Bacillus subtilis.

Spo0A-Dependent Activation of an Extended −10 Region Promoter in Bacillus subtilis

Mutational Analysis of the phoD Promoter in Bacillus subtilis: Implications for PhoP Binding and Promoter Activation of Pho Regulon Promoters

Two Regions of GerE Required for Promoter Activation in Bacillus subtilis