NDLI: Photoacoustic photocalorimetry and spectroscopy of Halobacterium halobium purple membranes.

Content Provider	PubMed Central
Author	Garty, H. Caplan, S. R. Cahen, D.
Abstract	Enthalpy changes associated with intermediates of the photocycle of bacteriorhodopsin (bR) in light-adapted Halobacterium halobium purple membranes, and decay times of these intermediates, are obtained from photoacoustic measurements on purple membrane fragments. Our results, mainly derived from modulation frequency spectra, show changes in the amount of energy stored in the intermediates and in their decay times as a function of pH and/or salt concentration. Especially affected are the slowest step (endothermic) and a spectroscopically unidentified intermediate (both at pH 7). This effect is interpreted in terms of cation binding to the protein, conformational changes of which are thought to be connected with the endothermic process. Wavelength spectra are used to obtain heat dissipation spectra, which allow identification of wavelength regions with varying photoactivity, and estimation of the amounts of enthalpy stored in the photointermediates. Because of bleaching and accumulation of intermediates, however, and because of the small fraction of light energy stored during photocycle, quantitative information cannot be obtained. From photoacoustic wavelength spectra of purple membrane fragments equilibrated at 63% relative humidity, rise and decay times of the bR570 and M412 intermediates are calculated.
Related Links	http://dx.doi.org/10.1016/s0006-3495(82)84686-9
Ending Page	415
Page Count	11
Starting Page	405
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	2
Volume Number	37
Language	English
Publisher Date	1982-02-01
Access Restriction	Open
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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Photoelectric signals from dried oriented purple membranes of Halobacterium halobium.

Electric dichroism in the purple membrane of Halobacterium halobium.

Energy transfer in the purple membrane of Halobacterium halobium.

Dehydration-Induced Molecular Structural Changes of Purple Membrane of Halobacterium Halobium

Effects of light adaptation on the purple membrane structure of Halobacterium halobium.

Contrasting molecular dynamics in red and purple membrane fractions of the Halobacterium halobium.

The quantum efficiency of proton pumping by the purple membrane of Halobacterium halobium.

Electro-optical measurements on aqueous suspension of purple membrane from Halobacterium halobium.

Effects of Bleaching and Regeneration on the Purple Membrane Structure of Halobacterium halobium

Photoacoustic photocalorimetry and spectroscopy of Halobacterium halobium purple membranes.

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Photoelectric signals from dried oriented purple membranes of Halobacterium halobium.

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Dehydration-Induced Molecular Structural Changes of Purple Membrane of Halobacterium Halobium

Effects of light adaptation on the purple membrane structure of Halobacterium halobium.

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Photoacoustic photocalorimetry and spectroscopy of Halobacterium halobium purple membranes.