NDLI: Genome-wide distribution of DNA replication origins at A+T-rich islands in Schizosaccharomyces pombe

Content Provider	PubMed Central
Author	Segurado, Mónica Luis, Alberto De Antequera, Francisco
Copyright Year	2003
Abstract	Genome-wide analysis of replication dynamics requires the previous identification of DNA replication origins (ORIs). However, variability among the ORIs makes it difficult to predict their distribution across the genome on the basis of their sequence. We report here that ORIs in Schizosaccharomyces pombe coincide with discrete chromosomal A+T-rich islands of up to 1 kb long that are characterized by a distinctive A+T content that clearly differentiates them from the rest of the genome. Genome-wide analysis has enabled us to identify 384 of these regions, which predicts the position of most ORIs in the genome, as shown by functional replication analyses. A+T-rich islands occur at the mating locus, centromeres and subtelomeric regions at a density that is approximately fourfold higher than elsewhere in the genome, which suggests a link between the origin recognition complex (ORC) and transcriptional silencing in these regions. The absence of consensus elements in A+T-rich islands implies that different sequences can target the ORC to different ORIs.
Related Links	http://dx.doi.org/10.1038/sj.embor.7400008
Ending Page	1053
Page Count	6
Starting Page	1048
File Format	PDF
ISSN	1469221X
e-ISSN	14693178
Journal	EMBO Reports
Issue Number	11
Volume Number	4
Language	English
Publisher Date	2003-11-01
Access Restriction	Open
Subject Keyword	Genetics Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Biochemistry Molecular Biology

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