NDLI: Involvement of KU80 in T-DNA integration in plant cells

Content Provider	PubMed Central
Author	Li, Jianxiong Vaidya, Manjusha White, Charles Alexander, Vainstein Citovsky, Vitaly Tzvi, Tzfira
Copyright Year	2005
Abstract	In Agrobacterium-mediated genetic transformation of plant cells, the bacterium exports a well defined transferred DNA (T-DNA) fragment and a series of virulence proteins into the host cell. Following its nuclear import, the single-stranded T-DNA is stripped of its escorting proteins, most likely converts to a double-stranded (ds) form, and integrates into the host genome. Little is known about the precise mechanism of T-DNA integration in plants, and no plant proteins specifically associated to T-DNA have been identified. Here we report the direct involvement of KU80, a protein that binds dsT-DNA intermediates. We show that ku80-mutant Arabidopsis plants are defective in T-DNA integration in somatic cells, whereas KU80-overexpressing plants exhibit increased susceptibility to Agrobacterium infection and increased resistance to DNA-damaging agents. The direct interaction between dsT-DNA molecules and KU80 in planta was confirmed by immunoprecipitation of KU80 dsT-DNA complexes from Agrobacterium-infected plants. Transformation of KU80-overexpressing plants with two separate T-DNA molecules resulted in an increased rate of extrachromosomal T-DNA to T-DNA recombination, indicating that KU80 bridges between dsT-DNAs and double-strand breaks. This last result further supports the notion that integration of T-DNA molecules occurs through ds intermediates and requires active participation of the host's nonhomologous end-joining repair machinery.
Related Links	http://dx.doi.org/10.1073/pnas.0506437103
Ending Page	19236
Page Count	6
Starting Page	19231
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	52
Volume Number	102
Language	English
Publisher	National Academy of Sciences
Publisher Date	2005-12-27
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Involvement of KU80 in T-DNA integration in plant cells.

Characterization of T-Circles and Their Formation Reveal Similarities to Agrobacterium T-DNA Integration Patterns

DNA repair protein Ku80 suppresses chromosomal aberrations and malignant transformation

Integration of Agrobacterium tumefaciens transfer DNA (T-DNA) involves rearrangements of target plant DNA sequences

“Agrolistic” transformation of plant cells: Integration of T-strands generated in planta

T-DNA integration into the barley genome from single and double cassette vectors

Arabidopsis mutants deficient in T-DNA integration.

Chromosomal double-strand break repair in Ku80-deficient cells.

Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA.

Involvement of KU80 in T-DNA integration in plant cells

Similar Documents

Involvement of KU80 in T-DNA integration in plant cells.

Characterization of T-Circles and Their Formation Reveal Similarities to Agrobacterium T-DNA Integration Patterns

DNA repair protein Ku80 suppresses chromosomal aberrations and malignant transformation

Integration of Agrobacterium tumefaciens transfer DNA (T-DNA) involves rearrangements of target plant DNA sequences

“Agrolistic” transformation of plant cells: Integration of T-strands generated in planta

T-DNA integration into the barley genome from single and double cassette vectors

Arabidopsis mutants deficient in T-DNA integration.

Chromosomal double-strand break repair in Ku80-deficient cells.

Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA.

Involvement of KU80 in T-DNA integration in plant cells