NDLI: Acute Ozone-Induced Differential Gene Expression Profiles in Rat Lung

Content Provider	PubMed Central
Author	Nadadur, Srikanth S. Costa, Daniel L. Slade, Ralph Robert, Silbjoris Hatch, Gary E.
Copyright Year	2005
Abstract	Ozone (O3) is an oxidant gas that can directly induce lung injury. Knowledge of the initial molecular events of the acute O3 response would be useful in developing biomarkers of exposure or response. Toward this goal, we exposed rats to toxic concentrations of O3 (2 and 5 ppm) for 2 hr and the molecular changes were assessed in lung tissue 2 hr postexposure using a rat cDNA expression array containing 588 characterized genes. Gene array analysis indicated differential expression in almost equal numbers of genes for the two exposure groups: 62 at 2 ppm and 57 at 5 ppm. Most of these genes were common to both exposure groups, suggesting common roles in the initial toxicity response. However, we also identified the induction of nine genes specific to 2-ppm (thyroid hormone-β receptor c-erb-A-β and glutathione reductase) or 5-ppm exposure groups (c-jun, induced nitric oxide synthase, macrophage inflammatory protein-2, and heat shock protein 27). Injury markers in bronchoalveolar lavage fluid (BALF) were used to assess immediate toxicity and inflammation in rats similarly exposed. At 2 ppm, injury was marked by significant increases in BALF total protein, N-acetylglucosaminidase, and lavageable ciliated cells. Because infiltration of neutrophils was observed only at the higher 5 ppm concentration, the distinctive genes suggested a potential amplification role for inflammation in the gene profile. Although the specific gene interactions remain unclear, this is the first report indicating a dose-dependent direct and immediate induction of gene expression that may be separate from those genes involved in inflammation after acute O3 exposure.
Related Links	http://dx.doi.org/10.1289/ehp.7413
Ending Page	1722
Page Count	6
Starting Page	1717
File Format	PDF
ISSN	00916765
e-ISSN	15529924
Journal	Environmental Health Perspectives
Issue Number	12
Volume Number	113
Language	English
Publisher	National Institute of Environmental Health Sciences
Publisher Date	2005-06-01
Access Restriction	Open
Rights Holder	National Institute of Environmental Health Sciences
Subject Keyword	Public Health, Environmental and Occupational Health Health, Toxicology and Mutagenesis Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health

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