NDLI: Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts

Content Provider	PubMed Central
Author	Ebert, A. M. Hume, G. L. Warren, K. S. Cook, N. P. Burns, C. G. Mohideen, M. A. Siegal, G. Yelon, D. Fishman, M. C. Garrity, D. M.
Copyright Year	2005
Abstract	Calcium entry into myocytes drives contraction of the embryonic heart. To prepare for the next contraction, myocytes must extrude calcium from intracellular space via the Na+/Ca2+ exchanger (NCX1) or sequester it into the sarcoplasmic reticulum, via the sarcoplasmic reticulum Ca2+-ATPase2 (SERCA2). In mammals, defective calcium extrusion correlates with increased intracellular calcium levels and may be relevant to heart failure and sarcoplasmic dysfunction in adults. We report here that mutation of the cardiac-specific NCX1 (NCX1h) gene causes embryonic lethal cardiac arrhythmia in zebrafish tremblor (tre) embryos. The tre ventricle is nearly silent, whereas the atrium manifests a variety of arrhythmias including fibrillation. Calcium extrusion defects in tre mutants correlate with severe disruptions in sarcomere assembly, whereas mutations in the L-type calcium channel that abort calcium entry do not produce this phenotype. Knockdown of SERCA2 activity by morpholino-mediated translational inhibition or pharmacological inhibition causes embryonic lethality due to defects in cardiac contractility and morphology but, in contrast to tre mutation, does not produce arrhythmia. Analysis of intracellular calcium levels indicates that homozygous tre embryos develop calcium overload, which may contribute to the degeneration of cardiac function in this mutant. Thus, the inhibition of NCX1h versus SERCA2 activity differentially affects the pathophysiology of rhythm in the developing heart and suggests that relative levels of NCX1 and SERCA2 function are essential for normal development.
Related Links	http://dx.doi.org/10.1073/pnas.0502683102
Ending Page	17710
Page Count	6
Starting Page	17705
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	49
Volume Number	102
Language	English
Publisher	National Academy of Sciences
Publisher Date	2005-12-06
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts.

The W-Loop of Alpha-Cardiac Actin Is Critical for Heart Function and Endocardial Cushion Morphogenesis in Zebrafish

Biphasic role for Wnt/β-catenin signaling in cardiac specification in zebrafish and embryonic stem cells

Cardiac sodium channel regulator MOG1 regulates cardiac morphogenesis and rhythm

Mutation in sodium–calcium exchanger 1 (NCX1) causes cardiac fibrillation in zebrafish

Filamin A (FLNA) is required for cell–cell contact in vascular development and cardiac morphogenesis

PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts

Bicoid cooperative DNA binding is critical for embryonic patterning in Drosophila

Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation

Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts

Similar Documents

Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts.

The W-Loop of Alpha-Cardiac Actin Is Critical for Heart Function and Endocardial Cushion Morphogenesis in Zebrafish

Biphasic role for Wnt/β-catenin signaling in cardiac specification in zebrafish and embryonic stem cells

Cardiac sodium channel regulator MOG1 regulates cardiac morphogenesis and rhythm

Mutation in sodium–calcium exchanger 1 (NCX1) causes cardiac fibrillation in zebrafish

Filamin A (FLNA) is required for cell–cell contact in vascular development and cardiac morphogenesis

PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts

Bicoid cooperative DNA binding is critical for embryonic patterning in Drosophila

Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation

Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts