NDLI: K+ Currents Activated by Depolarization in Cardiac Fibroblasts

Content Provider	PubMed Central
Author	Yoshiyuki, Shibukawa Chilton, E. Lisa Andrew, Maccannell K. Clark, Robert B. Giles, Wayne R.
Copyright Year	2005
Abstract	K+ currents expressed in freshly dispersed rat ventricular fibroblasts have been studied using whole-cell patch-clamp recordings. Depolarizing voltage steps from a holding potential of −90 mV activated time- and voltage-dependent outward currents at membrane potentials positive to ∼−30 mV. The relatively slow activation kinetics exhibited strong dependence on the membrane potential. Selected changes in extracellular K+ concentration ([K+]o) revealed that the reversal potentials of the tail currents changed as expected for a K+ equilibrium potential. The activation and inactivation kinetics of this K+ current, as well as its recovery from inactivation, were well-fitted by single exponential functions. The steady-state inactivation was well described by a Boltzmann function with a half-maximal inactivation potential (V 0.5) of −24 mV. Increasing [K+]o (from 5 to 100 mM) shifted this V 0.5 in the hyperpolarizing direction by −11 mV. Inactivation was slowed by increasing [K+]o to 100 mM, and the rate of recovery from inactivation was decreased after increasing [K+]o. Block of this K+ current by extracellular tetraethylammonium also slowed inactivation. These [K+]o-induced changes and tetraethylammonium effects suggest an important role for a C-type inactivation mechanism. This K+ current was sensitive to dendrotoxin-I (100 nM) and rTityustoxin Kα (50 nM).
Related Links	http://dx.doi.org/10.1529/biophysj.104.054429
Ending Page	3935
Page Count	12
Starting Page	3924
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	6
Volume Number	88
Language	English
Publisher	Biophysical Society
Publisher Date	2005-06-01
Access Restriction	Open
Rights Holder	Biophysical Society
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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