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| Content Provider | PubMed Central |
|---|---|
| Author | Haddadian, Esmael J. Gross, Elizabeth L. |
| Copyright Year | 2005 |
| Abstract | Using Brownian dynamics simulations, all of the charged residues in Chlamydomonas reinhardtii cytochrome c 6 (cyt c 6) and plastocyanin (PC) were mutated to alanine and their interactions with cytochrome f (cyt f) were modeled. Systematic mutation of charged residues on both PC and cyt c 6 confirmed that electrostatic interactions (at least in vitro) play an important role in bringing these proteins sufficiently close to cyt f to allow hydrophobic and van der Waals interactions to form the final electron transfer-active complex. The charged residue mutants on PC and cyt c 6 displayed similar inhibition classes. Our results indicate a difference between the two acidic clusters on PC. Mutations D44A and E43A of the lower cluster showed greater inhibition than do any of the mutations of the upper cluster residues. Replacement of acidic residues on cyt c 6 that correspond to the PC's lower cluster, particularly E70 and E69, was observed to be more inhibitory than those corresponding to the upper cluster. In PC residues D42, E43, D44, D53, D59, D61, and E85, and in cyt c 6 residues D2, E54, K57, D65, R66, E70, E71, and the heme had significant electrostatic contacts with cyt f charged residues. PC and cyt c 6 showed different binding sites and orientations on cyt f. As there are no experimental cyt c 6 mutation data available for algae, our results could serve as a good guide for future experimental work on this protein. The comparison between computational values and the available experimental data (for PC-cyt f interactions) showed overall good agreement, which supports the predictive power of Brownian dynamics simulations in mutagenesis studies. |
| Related Links | http://dx.doi.org/10.1529/biophysj.104.053561 |
| Ending Page | 2339 |
| Page Count | 17 |
| Starting Page | 2323 |
| File Format | |
| ISSN | 00063495 |
| e-ISSN | 15420086 |
| Journal | Biophysical Journal |
| Issue Number | 3 |
| Volume Number | 88 |
| Language | English |
| Publisher | Biophysical Society |
| Publisher Date | 2005-03-01 |
| Access Restriction | Open |
| Rights Holder | Biophysical Society |
| Subject Keyword | Biophysics Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biophysics |
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