NDLI: Myosin Regulatory Light Chain Phosphorylation and Strain Modulate Adenosine Diphosphate Release from Smooth Muscle Myosin

Content Provider	PubMed Central
Author	Khromov, Alexander S. Webb, Martin R. Ferenczi, Michael A. Trentham, David R. Somlyo, Andrew P. Somlyo, Avril V.
Copyright Year	2004
Abstract	The effects of myosin regulatory light chain (RLC) phosphorylation and strain on adenosine diphosphate (ADP) release from cross-bridges in phasic (rabbit bladder (Rbl)) and tonic (femoral artery (Rfa)) smooth muscle were determined by monitoring fluorescence transients of the novel ADP analog, 3′-deac-eda-ADP (deac-edaADP). Fluorescence transients reporting release of 3′-deac-eda-ADP were significantly faster in phasic (0.57 ± 0.06 s−1) than tonic (0.29 ± 0.03 s−1) smooth muscles. Thiophosphorylation of regulatory light chains increased and strain decreased the release rate ∼twofold. The calculated (k −ADP/k +ADP) dissociation constant, K d of unstrained, unphosphorylated cross-bridges for ADP was 0.6 μM for rabbit bladder and 0.3 μM for femoral artery. The rates of ADP release from rigor bridges and reported values of Pi release (corresponding to the steady-state adenosine triphosphatase (ATPase) rate of actomyosin (AM)) from cross-bridges during a maintained isometric contraction are similar, indicating that the ADP-release step or an isomerization preceding it may be limiting the adenosine triphosphatase rate. We conclude that the strain- and dephosphorylation-dependent high affinity for and slow ADP release from smooth muscle myosin prolongs the fraction of the duty cycle occupied by strongly bound actomyosin.ADP state(s) and contributes to the high economy of force.
Starting Page	2318
File Format	PDF
ISSN	15420086
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	4
Volume Number	86
Language	English
Publisher	Biophysical Society
Publisher Date	2004-04-01
Access Restriction	Open
Rights Holder	Biophysical Society
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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