NDLI: Photo-Oxidation of P740, the Primary Electron Donor in Photosystem I from Acaryochloris marina

Content Provider	PubMed Central
Author	Sivakumar, Velautham Wang, Ruili Hastings, Gary
Copyright Year	2003
Abstract	Fourier transform infrared spectroscopy (FTIR) difference spectroscopy in combination with deuterium exchange experiments has been used to study the photo-oxidation of P740, the primary electron donor in photosystem I from Acaryochloris marina. Comparison of (P740+-P740) and (P700+-P700) FTIR difference spectra show that P700 and P740 share many structural similarities. However, there are several distinct differences also: 1), The (P740+-P740) FTIR difference spectrum is significantly altered upon proton exchange, considerably more so than the (P700+-P700) FTIR difference spectrum. The P740 binding pocket is therefore more accessible than the P700 binding pocket. 2), Broad, “dimer” absorption bands are observed for both P700+ and P740+. These bands differ significantly in substructure, however, suggesting differences in the electronic organization of P700+ and P740+. 3), Bands are observed at 2727(−) and 2715(−) cm−1 in the (P740+-P740) FTIR difference spectrum, but are absent in the (P700+-P700) FTIR difference spectrum. These bands are due to formyl CH modes of chlorophyll d. Therefore, P740 consists of two chlorophyll d molecules. Deuterium-induced modification of the (P740+-P740) FTIR difference spectrum indicates that only the highest frequency 133 ester carbonyl mode of P740 downshifts, indicating that this ester mode is weakly H-bonded. In contrast, the highest frequency ester carbonyl mode of P700 is free from H-bonding. Deuterium-induced changes in (P740+-P740) FTIR difference spectrum could also indicate that one of the chlorophyll d 31 carbonyls of P740 is hydrogen bonded.
Related Links	http://dx.doi.org/10.1016/s0006-3495(03)74734-1
Ending Page	3172
Page Count	11
Starting Page	3162
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	5
Volume Number	85
Language	English
Publisher	Biophysical Society
Publisher Date	2003-11-01
Access Restriction	Open
Rights Holder	Biophysical Society
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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