NDLI: A genetically targetable fluorescent probe of channel gating with rapid kinetics.

Content Provider	PubMed Central
Author	Kazuto, Ataka Pieribone, Vincent A.
Abstract	We have developed a genetically targetable, optical channel-gating reporter that converts rapid membrane potential changes into changes in fluorescence intensity. We have named this construct SPARC (sodium channel protein-based activity reporting construct). Green fluorescent protein was inserted into an intracellular loop of a reversibly nonconducting form of the rat mu I skeletal muscle voltage-gated sodium channel. Rapid changes of the membrane potential modulate the fluorescence of the inserted green fluorescent protein. This change in fluorescence can faithfully report depolarizing pulses as short as 2 ms. The fluorescence signal does not inactivate during extended depolarizations. Several features of the probe's response properties indicate that it likely reports gating charge movement of a single domain of rat mu I skeletal muscle. This probe provides a new approach for studying rapid channel movements and may possibly act as a fluorescent activity reporter in excitable cells.
Related Links	http://dx.doi.org/10.1016/s0006-3495(02)75415-5
Ending Page	516
Page Count	8
Starting Page	509
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	1 Pt 1
Volume Number	82
Language	English
Publisher Date	2002-01-01
Access Restriction	Open
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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A genetically targetable fluorescent probe of channel gating with rapid kinetics.