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| Content Provider | PubMed Central |
|---|---|
| Author | Huang, Xiaohong Xuan, Xuenan Suzuki, Hiroshi Sugimoto, Chihiro Nagasawa, Hideyuki Fujisaki, Kozo Mikami, Takeshi Igarashi, Ikuo |
| Copyright Year | 2002 |
| Abstract | A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs infected with T. gondii. Mice immunized with S-rSAG2 produced high titers of specific immunoglobulin G2a (IgG2a) and IgG1 antibodies. In an indirect fluorescent antibody test, all mouse antisera against S-rSAG2 reacted strongly to the natural parasites, but those against rSAG2 expressed in Escherichia coli (E-rSAG2) only showed very weak reaction, although no markedly difference was found in the reaction to denatured antigen, T. gondii lysate, in Western blot analysis. The results suggest that S-rSAG2 is better than E-rSAG2 in both antigenicity and immunogenicity. Enzyme-linked immunosorbent assay (ELISA) with S-rSAG2 could differentiate clearly between sera from 30 specific-pathogen-free cats and 4 experimentally infected cats. Serum samples from domestic cats in Japan were tested by the ELISA and compared with a latex agglutination test (LAT) and ELISA with E-rSAG2. Of 187 samples, all 35 LAT-positive sera had strong reactions to S-rSAG2 and E-rSAG2. Of the 152 LAT-negative sera, 18 were positive in the ELISA with S-rSAG2, whereas only 2 were positive in the ELISA with E-rSAG2. Although there were significant correlations among the three methods, the ELISA with S-rSAG2 was more sensitive than the others, which could be attributed to the fact that S-rSAG2 shares some common conformational structure with the native antigen. The results suggest that S-rSAG2 would be a useful reagent for the detection of T. gondii infection in cats. |
| Related Links | http://dx.doi.org/10.1128/cdli.9.6.1343-1347.2002 |
| Ending Page | 1347 |
| Page Count | 5 |
| Starting Page | 1343 |
| File Format | |
| ISSN | 15566811 |
| e-ISSN | 10986588 |
| Journal | Clinical and Diagnostic Laboratory Immunology |
| Issue Number | 6 |
| Volume Number | 9 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2002-11-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Immunology Immunology and Allergy Microbiology (medical) Clinical Biochemistry Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Clinical Biochemistry Immunology Microbiology (medical) |
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