NDLI: Site-specific thermodynamics and kinetics of a coiled-coil transition by spin inversion transfer NMR.

Content Provider	PubMed Central
Author	D'avignon, D. A. Bretthorst, G. L. Holtzer, M. E. Holtzer, A.
Abstract	A 33-residue pseudo-wild-type GCN4 leucine zipper peptide is used to probe the equilibrium conformational population in proteins. 13Calpha-NMR shows that chain sites differ in structural content at a given temperature, and that two dimeric folded forms are evident at many sites. Spin inversion transfer experiments are reported bearing on the thermodynamics and kinetics of interconversion of the two dimeric folded forms (Fa <--> Fb) at the 13Calpha-labeled position L13. At each temperature, at conditions wherein the population of unfolded chains is quite small, inversion of the Fa spins via a tuned Gaussian pi-pulse is followed by a time interval (tau), interrogation, and recording of the free induction decay. Fifteen such inversions, with varying tau, provide the time course for recovery of equilibrium magnetization after inversion. Similar experiments follow inversion of the Fb spins. Re-equilibration is known to be modulated by four first-order rate constants: two (T1a(-1) and T1b(-1)) for spin-lattice relaxation intrinsic to the respective sites, and two (kab and kba) for the conformational change. All four follow from joint, Bayesian analysis of all the data at each temperature. The equilibrium constant at each temperature for this local transition, determined simply from the equilibrium relative magnetizations at Fa and Fb sites, agrees well with the kinetic ratio kab/kba. The standard Gibbs energies, enthalpy, and entropy follow. Activation parameters, both ways, are accessible from the rate constants and suggest a transition state with high Gibbs energy and enthalpy, but with entropy between those of Fa and Fb.
Related Links	http://dx.doi.org/10.1016/s0006-3495(98)78025-7
Ending Page	3197
Page Count	8
Starting Page	3190
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	6
Volume Number	74
Language	English
Publisher Date	1998-06-01
Access Restriction	Open
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

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