NDLI: Pre-spliceosome formation in S.pombe requires a stable complex of SF1–U2AF59

Content Provider	PubMed Central
Author	Huang, Tao Vilardell, Josep Query, Charles C.
Copyright Year	2002
Abstract	We have initiated a biochemical analysis of splicing complexes in extracts from the fission yeast Schizosaccharomyces pombe. Extracts of S.pombe contain high levels of the spliceosome-like U2/5/6 tri-snRNP, which dissociates into mono-snRNPs in the presence of ATP, and supports binding of U2 snRNP to the 3′ end of introns, yielding a weak ATP-independent E complex and the stable ATP-dependent complex A. The requirements for S.pombe complex A formation (pre-mRNA sequence elements, protein splicing factors, SF1/BBP and both subunits of U2AF) are analogous to those of mammalian complex A. The S.pombe SF1/BBP, U2AF59 and U2AF23 are tightly associated in a novel complex that is required for complex A formation. This pre-formed SF1– U2AF59–U2AF23 complex may represent a streamlined mechanism for recognition of the branch site, pyrimidine tract and 3′ splice site at the 3′ end of introns.
Related Links	http://dx.doi.org/10.1093/emboj/cdf555
Starting Page	5516
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	20
Volume Number	21
Language	English
Publisher	Oxford University Press
Publisher Date	2002-10-15
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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Pre-spliceosome formation in S.pombe requires a stable complex of SF1–U2AF59–U2AF23