NDLI: DNA damage-induced apoptosis requires the DNA-dependent protein kinase, and is mediated by the latent population of p53

Content Provider	PubMed Central
Author	Woo, Richard A. Jack, Melissa T. Xu, Yang Sandeep, Burma Chen, David J. Lee, Patrick W. K.
Copyright Year	2002
Abstract	Mouse embryo fibroblasts (MEFs) expressing the adenovirus E1A protein undergo apoptosis upon exposure to ionizing radiation. We show here that immediately following γ-irradiation, latent p53 formed a complex with the catalytic subunit of the DNA-dependent protein kinase (DNA-PKCS). The complex formation was DNase sensitive, suggesting that the proteins came together on the DNA, conceivably at strand breaks. This association was accompanied by phosphorylation of pre-existing, latent p53 at Ser18 (corresponding to Ser15 in human p53), which was not found in DNA-PKCS –/– cells. Most significantly, DNA damage-induced apoptosis was abolished in both DNA-PKCS –/– and p53–/– cells. In addition, blocking synthesis of inducible p53 by cycloheximide did not abrogate apoptosis, suggesting that the latent population of p53 is sufficient for executing the apoptotic program. Finally, E1A-expressing MEFs from a p53 ‘knock-in’ mouse where Ser18 was mutated to an alanine had an attenuated apoptotic response, indicating that phosphorylation of this site by DNA-PK is a contributing factor for apoptosis.
Related Links	http://dx.doi.org/10.1093/emboj/cdf307
Ending Page	3008
Page Count	9
Starting Page	3000
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	12
Volume Number	21
Language	English
Publisher	Oxford University Press
Publisher Date	2002-06-17
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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