NDLI: A novel family of RNA tetraloop structure forms the recognition site for Saccharomyces cerevisiae RNase III

Content Provider	PubMed Central
Author	Wu, Haihong Yang, Pok K. Butcher, Samuel E. Kang, Sundeep Chanfreau, Guillaume Feigon, Juli
Copyright Year	2001
Abstract	RNases III are a family of double-stranded RNA (dsRNA) endoribonucleases involved in the processing and decay of a large number of cellular RNAs as well as in RNA interference. The dsRNA substrates of Saccharomyces cerevisiae RNase III (Rnt1p) are capped by tetraloops with the consensus sequence AGNN, which act as the primary docking site for the RNase. We have solved the solution structures of two RNA hairpins capped by AGNN tetraloops, AGAA and AGUU, using NMR spectroscopy. Both tetraloops have the same overall structure, in which the backbone turn occurs on the 3′ side of the syn G residue in the loop, with the first A and G in a 5′ stack and the last two residues in a 3′ stack. A non-bridging phosphate oxygen and the universal G which are essential for Rnt1p binding are strongly exposed. The compared biochemical and structural analysis of various tetraloop sequences defines a novel family of RNA tetraloop fold with the consensus (U/A)GNN and implicates this conserved structure as the primary determinant for specific recognition of Rnt1p substrates.
Related Links	http://dx.doi.org/10.1093/emboj/20.24.7240
Ending Page	7249
Page Count	10
Starting Page	7240
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	24
Volume Number	20
Language	English
Publisher	Oxford University Press
Publisher Date	2001-12-17
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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