NDLI: End invasion of peptide nucleic acids (PNAs) with mixed-base composition into linear DNA duplexes

Content Provider	PubMed Central
Author	Smolina, Irina V. Demidov, Vadim V. Soldatenkov, Viatcheslav A. Chasovskikh, Sergey G. Frank-kamenetskii, Maxim D.
Copyright Year	2005
Abstract	Peptide nucleic acid (PNA) is a synthetic DNA mimic with valuable properties and a rapidly growing scope of applications. With the exception of recently introduced pseudocomplementary PNAs, binding of common PNA oligomers to target sites located inside linear double-stranded DNAs (dsDNAs) is essentially restricted to homopurine–homopyrimidine sequence motifs, which significantly hampers some of the PNA applications. Here, we suggest an approach to bypass this limitation of common PNAs. We demonstrate that PNA with mixed composition of ordinary nucleobases is capable of sequence-specific targeting of complementary dsDNA sites if they are located at the very termini of DNA duplex. We then show that such targeting makes it possible to perform capturing of designated dsDNA fragments via the DNA-bound biotinylated PNA as well as to signal the presence of a specific dsDNA sequence, in the case a PNA beacon is employed. We also examine the PNA–DNA conjugate and prove that it can initiate the primer-extension reaction starting from the duplex DNA termini when a DNA polymerase with the strand-displacement ability is used. We thus conclude that recognition of duplex DNA by mixed-base PNAs via the end invasion has a promising potential for site-specific and sequence-unrestricted DNA manipulation and detection.
Related Links	http://dx.doi.org/10.1093/nar/gni151
Starting Page	146
File Format	PDF
ISSN	03051048
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	17
Volume Number	33
Language	English
Publisher	Oxford University Press
Publisher Date	2005-09-25
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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End invasion of peptide nucleic acids (PNAs) with mixed-base composition into linear DNA duplexes

End invasion of peptide nucleic acids (PNAs) with mixed-base composition into linear DNA duplexes

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