NDLI: The effects of external pH on calcium channel currents in bullfrog sympathetic neurons.

Content Provider	PubMed Central
Author	Zhou, W. Jones, S. W.
Abstract	We have investigated the effects of external pH (pHo) on whole-cell calcium channel currents in bullfrog sympathetic neurons. The peak inward current increased at alkaline pHo and decreased at acidic pHo. We used tail currents to distinguish effects of pHo on channel gating and permeation. There were large shifts in the voltage dependence of channel activation (approximately 40 mV between pHo and 9.0 and pHo 5.6), which could be explained by binding of H+ to surface charge according to Gouy-Chapman theory. To examine the effects of pHo on permeation, we measured tail currents at 0 mV, following steps to + 120 mV to maximally activate the channels. Unlike most previous studies, we found only a approximately 10% reduction in channel conductance from pHo 9.0 to pHo 6.4, despite a approximately 25 mV shift of channel activation. At lower pHo the channel conductance did decrease, which could be described by binding of H+ to a site with pKa = 5.1. In some cells, there was a separate slow decrease in conductance at low pHo, possibly because of changes in internal pH. These results suggest that changes in current at pHo > 6.4 result primarily from a shift in the voltage dependence of channel activation. A H(+)-binding site can explain a rapid decrease in channel conductance at lower pHo. The surface charge affecting gating has little effect on the local ion concentration near the pore, or on the channel conductance.
Related Links	http://dx.doi.org/10.1016/s0006-3495(96)79689-3
Ending Page	1334
Page Count	9
Starting Page	1326
File Format	PDF
ISSN	00063495
e-ISSN	15420086
Journal	Biophysical Journal
Issue Number	3
Volume Number	70
Language	English
Publisher Date	1996-03-01
Access Restriction	Open
Subject Keyword	Biophysics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Calcium currents in bullfrog sympathetic neurons. II. Inactivation

Calcium currents in bullfrog sympathetic neurons. I. Activation kinetics and pharmacology

Surface charge and calcium channel saturation in bullfrog sympathetic neurons

Effects of temperature on calcium current of bullfrog sympathetic neurons.

Surface charge and lanthanum block of calcium current in bullfrog sympathetic neurons.

Effects of arachidonic acid on unitary calcium currents in rat sympathetic neurons

Gating of Single N-type Calcium Channels Recorded from Bullfrog Sympathetic Neurons

Bile Acid Inhibition of N-type Calcium Channel Currents from Sympathetic Ganglion Neurons

M-currents and other potassium currents in bullfrog sympathetic neurones

The effects of external pH on calcium channel currents in bullfrog sympathetic neurons.

Similar Documents

Calcium currents in bullfrog sympathetic neurons. II. Inactivation

Calcium currents in bullfrog sympathetic neurons. I. Activation kinetics and pharmacology

Surface charge and calcium channel saturation in bullfrog sympathetic neurons

Effects of temperature on calcium current of bullfrog sympathetic neurons.

Surface charge and lanthanum block of calcium current in bullfrog sympathetic neurons.

Effects of arachidonic acid on unitary calcium currents in rat sympathetic neurons

Gating of Single N-type Calcium Channels Recorded from Bullfrog Sympathetic Neurons

Bile Acid Inhibition of N-type Calcium Channel Currents from Sympathetic Ganglion Neurons

M-currents and other potassium currents in bullfrog sympathetic neurones

The effects of external pH on calcium channel currents in bullfrog sympathetic neurons.