NDLI: Characterization of SALMONELLA TYPHIMURIUM Mutants with Altered Glutamine Synthetase Activity

Content Provider	PubMed Central
Author	Funanage, Vicky L. Brenchley, Jean E.
Abstract	A number of glutamine auxotrophs of Salmonella typhimurium were isolated and characterized genetically. Three of the mutations appear to be closely linked and are complemented by episomes carrying the glnA region of Escherichia coli. The lesions in these strains are approximately 20% linked by P1 transduction with a mutation in the rha gene, but are unlinked to ilv. Another mutation causing glutamine auxotrophy in strain JB674 is genetically distinct from the others. Strain JB674 grown in glucose medium containing ammonia as the nitrogen source has reduced levels of glutamine synthetase that is more adenylylated than in the parent strain, suggesting that the enzyme can not be deadenylylated normally. The lesion causing glutamine auxotrophy in JB674 lies in the region corresponding to the glnB and glnE genes affecting glutamine synthetase modification in Klebsiella areogenes. Four Gln+ revertants of JB674 have glutamine synthetase activities 4 to 6 fold higher than normal. One mutation causing this increased enzyme synthesis has been shown by three-factor crosses with the glnA mutations to lie near or within the glnA gene.
Starting Page	513
File Format	PDF
ISSN	00166731
Journal	Genetics
Issue Number	3
Volume Number	86
Language	English
Publisher Date	1977-07-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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Characterization of SALMONELLA TYPHIMURIUM Mutants with Altered Glutamine Synthetase Activity