NDLI: Transcription of Gypsy Elements in a Y-Chromosome Male Fertility Gene of Drosophila Hydei

Content Provider	PubMed Central
Author	Hochstenbach, R. Harhangi, H. Schouren, K. Bindels, P. Suijkerbuijk, R. Hennig, W.
Abstract	We have found that defective gypsy retrotransposons are a major constituent of the lampbrush loop pair Nooses in the short arm of the Y chromosome of Drosophila hydei. The loop pair is formed by male fertility gene Q during the primary spermatocyte stage of spermatogenesis, each loop being a single transcription unit with an estimated length of 260 kb. Using fluorescent in situ hybridization, we show that throughout the loop transcripts gypsy elements are interspersed with blocks of a tandemly repetitive Y-specific DNA sequence, ay1. Nooses transcripts containing both sequence types show a wide size range on Northern blots, do not migrate to the cytoplasm, and are degraded just before the first meiotic division. Only one strand of ay1 and only the coding strand of gypsy can be detected in the loop transcripts. However, as cloned genomic DNA fragments also display opposite orientations of ay1 and gypsy, such DNA sections cannot be part of the Nooses. Hence, they are most likely derived from the flanking heterochromatin. The direction of transcription of ay1 and gypsy thus appears to be of a functional significance.
Starting Page	437
File Format	PDF
ISSN	00166731
Journal	Genetics
Issue Number	2
Volume Number	142
Language	English
Publisher Date	1996-02-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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Transcription of Gypsy Elements in a Y-Chromosome Male Fertility Gene of Drosophila Hydei