NDLI: NMR structural analysis of a peptide mimic of the bridging sheet of HIV-1 gp120 in methanol and water

Content Provider	PubMed Central
Author	Chakraborty, Kausik Shivakumar, P. Raghothama, S. Varadarajan, Raghavan
Copyright Year	2005
Abstract	gp120 is a subunit of the Env (viral envelope protein) of HIV-1. The protein consists of inner and outer domains linked by a bridging sheet. Several gp120 residues that bind the neutralizing antibody 17b as well as the cellular co-receptor CCR5 (CC chemokine receptor 5), are located in the bridging sheet. Peptides that mimic the 17b-binding regions of gp120 would be useful potential immunogens for the generation of neutralizing antibodies against HIV-1. Towards this end, a 26-residue, four-stranded β-sheet peptide was designed on the basis of the structure of the bridging sheet, and its structure was characterized in methanol by NMR. In methanol, amide and α-proton resonances were well resolved and dispersed. A number of interstrand NOEs (nuclear Overhauser effects) were observed, providing good evidence for multiple turn β-hairpin structure. NOEs also provided good evidence for all Xxx–D-Pro bonds in the trans configuration and all three turns formed by a two residue D-Pro–Gly segment to be of type II′ turn. The structure conforms well to the designed four-stranded β-sheet structure. Approx. 20% of the peptide was estimated to adopt a folded conformation in water, as evidenced by CD spectroscopy. This was consistent with smaller, but still significant, downfield shifts of CαH protons relative to random-coil values. A second peptide was designed with two disulphide bonds to further constrain the peptide backbone. While structured in methanol, this peptide, like the previous one, also exhibits only partial structure formation in water, as evidenced by CD spectroscopy.
Related Links	http://dx.doi.org/10.1042/bj20050442
Ending Page	581
Page Count	9
Starting Page	573
File Format	PDF
ISSN	02646021
e-ISSN	14708728
Journal	Biochemical Journal
Issue Number	Pt 2
Volume Number	390
Language	English
Publisher	Portland Press Ltd.
Publisher Date	2005-09-01
Access Restriction	Open
Rights Holder	Portland Press Ltd.
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Design and Characterization of a Peptide Mimotope of the HIV-1 gp120 Bridging Sheet

A model of the peptide triazole entry inhibitor binding to HIV-1 gp120 and mechanism of bridging sheet disruption

The conformation and orientation of a 27-residue CCR5-peptide in a ternary complex with HIV-1 gp120 and a CD4-mimic peptide †

Peptide Mimic of the HIV Envelope gp120-gp41 Interface

A peptide inhibitor of HIV-1 neutralizing antibody 2G12 is not a structural mimic of the natural carbohydrate epitope on gp120

Design and Characterization of a Peptide Mimotope of the HIV-1 gp120 Bridging Sheet

HIV-1 Env gp120 Structural Determinants for Peptide Triazole Dual Receptor Site Antagonism

Probing the HIV gp120 Envelope Glycoprotein Conformation by NMR*

Probing the HIV gp120 Envelope Glycoprotein Conformation by NMR

NMR structural analysis of a peptide mimic of the bridging sheet of HIV-1 gp120 in methanol and water

Similar Documents

Design and Characterization of a Peptide Mimotope of the HIV-1 gp120 Bridging Sheet

A model of the peptide triazole entry inhibitor binding to HIV-1 gp120 and mechanism of bridging sheet disruption

The conformation and orientation of a 27-residue CCR5-peptide in a ternary complex with HIV-1 gp120 and a CD4-mimic peptide †

Peptide Mimic of the HIV Envelope gp120-gp41 Interface

A peptide inhibitor of HIV-1 neutralizing antibody 2G12 is not a structural mimic of the natural carbohydrate epitope on gp120

Design and Characterization of a Peptide Mimotope of the HIV-1 gp120 Bridging Sheet

HIV-1 Env gp120 Structural Determinants for Peptide Triazole Dual Receptor Site Antagonism

Probing the HIV gp120 Envelope Glycoprotein Conformation by NMR*

Probing the HIV gp120 Envelope Glycoprotein Conformation by NMR

NMR structural analysis of a peptide mimic of the bridging sheet of HIV-1 gp120 in methanol and water