NDLI: Characterization of the Exosporium Basal Layer Protein BxpB of Bacillus anthracis

Content Provider	PubMed Central
Author	Steichen, Christopher T. Kearney, John F. Turnbough, Charles L.
Copyright Year	2005
Abstract	Bacillus anthracis spores, the cause of anthrax, are enclosed by a prominent loose-fitting structure called the exosporium. The exosporium is composed of a basal layer and an external hair-like nap. The filaments of the hair-like nap are apparently formed by a single collagen-like glycoprotein called BclA, whereas several different proteins form or are tightly associated with the basal layer. In this study, we used immunogold electron microscopy to demonstrate that BxpB (also called ExsF) is a component of the exosporium basal layer. Binding to the basal layer by an anti-BxpB monoclonal antibody was greatly increased by the loss of BclA. We found that BxpB and BclA are part of a stable complex that appears to include the putative basal layer protein ExsY and possibly other proteins. Previous results suggested that BxpB was glycosylated; however, our results indicate that it is not a glycoprotein. We showed that ΔbxpB spores, which lack BxpB, contain an exosporium devoid of hair-like nap even though the ΔbxpB strain produces normal levels of BclA. These results indicated that BxpB is required for the attachment of BclA to the exosporium. Finally, we found that the efficiency of production of ΔbxpB spores and their resistance properties were similar to those of wild-type spores. However, ΔbxpB spores germinate faster than wild-type spores, indicating that BxpB suppresses germination. This effect did not appear to be related to the absence from ΔbxpB spores of a hair-like nap or of enzymes that degrade germinants.
Related Links	http://dx.doi.org/10.1128/jb.187.17.5868-5876.2005
Ending Page	5876
Page Count	9
Starting Page	5868
File Format	PDF
ISSN	00219193
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	17
Volume Number	187
Language	English
Publisher	American Society for Microbiology
Publisher Date	2005-09-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Molecular Biology Microbiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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The Co-Dependency of BxpB/ExsFA and BclA for Proper Incorporation into the Exosporium of Bacillus anthracis

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