NDLI: A statherin and calcium enriched layer at the air interface of human parotid saliva

Content Provider	PubMed Central
Author	Proctor, Gordon B. Hamdan, Sawsan Carpenter, Guy H. Wilde, Peter
Copyright Year	2005
Abstract	Parotid saliva placed in 35-mm-diameter tissue culture dishes developed increasing surface viscoelasticity at the interface with air. A surface layer became visible with time, and was collected and analysed by protein electrophoresis which indicated that a single protein (pI 4.2; molecular mass approx. 6 kDa) predominated. Western blot analysis demonstrated that the major protein band reacted with an antiserum directed against the C-terminal of the calcium-binding salivary protein statherin. Matrix-assisted laser-desorption ionization–time-of-flight MS analysis gave a molecular mass of 5380 Da for the protein, corresponding to the molecular mass of statherin. Staining of film protein in electrophoresis gels was compared with statherin synthesized on a solid phase, and the mean statherin content of film formed from 1 ml of parotid saliva was measured as 7 nmol. The mean calcium content of the surface layer was 250 nmol. Surface rheology was greatly decreased in the presence of EDTA, whereas surface tension of saliva was unaffected by calcium chelation, suggesting that protein accumulated at the surface was unaffected. The results suggest that a layer rich in statherin forms at the interface of saliva and air, and that the surface rheology developed is dependent upon protein interactions mediated by calcium. The surface layer may enhance the function of saliva as a protective layer on the mucosal surfaces and teeth.
Related Links	http://dx.doi.org/10.1042/BJ20042012
Starting Page	111
File Format	PDF
ISSN	14708728
e-ISSN	14708728
Journal	Biochemical Journal
Issue Number	Pt 1
Volume Number	389
Language	English
Publisher	Portland Press Ltd.
Publisher Date	2005-07-01
Access Restriction	Open
Rights Holder	Portland Press Ltd.
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Complete covalent structure of statherin, a tyrosine-rich acidic peptide which inhibits calcium phosphate precipitation from human parotid saliva.

Chemical and physical characteristics of a phosphoprotein from human parotid saliva.

Calcium transport mechanisms in basolateral plasma membrane-enriched vesicles from rat parotid gland.

Purification and partial characterization of four proteins from human parotid saliva

THE PROTEIN CONTENT OF HUMAN PAROTID SALIVA

Fractionation of human parotid saliva proteins.

The identification of a peptide in human parotid saliva particularly active in enhancing the glycolytic activity of the salivary micro-organisms.

The binding of calcium to a salivary phosphoprotein, protein A, common to human parotid and submandibular secretions.

The hydrolysis of phosphatidylinositol monolayers at an air/water interface by the calcium-ion-dependent phosphatidylinositol phosphodiesterase of pig brain.

A statherin and calcium enriched layer at the air interface of human parotid saliva

Similar Documents

Complete covalent structure of statherin, a tyrosine-rich acidic peptide which inhibits calcium phosphate precipitation from human parotid saliva.

Chemical and physical characteristics of a phosphoprotein from human parotid saliva.

Calcium transport mechanisms in basolateral plasma membrane-enriched vesicles from rat parotid gland.

Purification and partial characterization of four proteins from human parotid saliva

THE PROTEIN CONTENT OF HUMAN PAROTID SALIVA

Fractionation of human parotid saliva proteins.

The identification of a peptide in human parotid saliva particularly active in enhancing the glycolytic activity of the salivary micro-organisms.

The binding of calcium to a salivary phosphoprotein, protein A, common to human parotid and submandibular secretions.

The hydrolysis of phosphatidylinositol monolayers at an air/water interface by the calcium-ion-dependent phosphatidylinositol phosphodiesterase of pig brain.

A statherin and calcium enriched layer at the air interface of human parotid saliva