NDLI: Actin Filament Bundles in Drosophila Wing Hairs: Hairs and Bristles Use Different Strategies for Assembly

Content Provider	PubMed Central
Author	Guild, Gregory M. Connelly, Patricia S. Ruggiero, Linda Vranich, Kelly A. Tilney, Lewis G.
Editor	Matsudaira, Paul
Copyright Year	2005
Abstract	Actin filament bundles can shape cellular extensions into dramatically different forms. We examined cytoskeleton formation during wing hair morphogenesis using both confocal and electron microscopy. Hairs elongate with linear kinetics (∼1 μm/h) over the course of ∼18 h. The resulting structure is vividly asymmetric and shaped like a rose thorn—elongated in the distal direction, curved in two dimensions with an oval base and a round tip. High-resolution analysis shows that the cytoskeleton forms from microvilli-like pimples that project actin filaments into the cytoplasm. These filaments become cross-linked into bundles by the sequential use of three cross-bridges: villin, forked and fascin. Genetic loss of each cross-bridge affects cell shape. Filament bundles associate together, with no lateral membrane attachments, into a cone of overlapping bundles that matures into an oval base by the asymmetric addition of bundles on the distal side. In contrast, the long bristle cell extension is supported by equally long (up to 400 μm) filament bundles assembled together by end-to-end grafting of shorter modules. Thus, bristle and hair cells use microvilli and cross-bridges to generate the common raw material of actin filament bundles but employ different strategies to assemble these into vastly different shapes.
Related Links	http://dx.doi.org/10.1091/mbc.e05-03-0185
Ending Page	3631
Page Count	12
Starting Page	3620
File Format	PDF
ISSN	10591524
Journal	Molecular Biology of the Cell
Issue Number	8
Volume Number	16
Language	English
Publisher	The American Society for Cell Biology
Publisher Date	2005-05-01
Access Restriction	Open
Rights Holder	The American Society for Cell Biology
Subject Keyword	Cell Biology Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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