NDLI: Structure, function and tissue forms of the C-terminal globular domain of collagen XVIII containing the angiogenesis inhibitor endostatin.

Content Provider	PubMed Central
Author	Sasaki, T. Fukai, N. Mann, K. Göhring, W. Olsen, B. R. Timpl, R.
Abstract	The C-terminal domain NC1 of mouse collagen XVIII (38 kDa) and the shorter mouse and human endostatins (22 kDa) were prepared in recombinant form from transfected mammalian cells. The NC1 domain aggregated non-covalently into a globular trimer which was partially cleaved by endogenous proteolysis into several monomers (25-32 kDa) related to endostatin. Endostatins were obtained in a highly soluble, monomeric form and showed a single N-terminal sequence which, together with other data, indicated a compact folding. Endostatins and NC1 showed a comparable binding activity for the microfibrillar fibulin-1 and fibulin-2, and for heparin. Domain NC1, however, was a distinctly stronger ligand than endostatin for sulfatides and the basement membrane proteins laminin-1 and perlecan. Immunological assays demonstrated endostatin epitopes on several tissue components (22-38 kDa) and in serum (120-300 ng/ml), the latter representing the smaller variants. The data indicated that the NC1 domain consists of an N-terminal association region (approximately 50 residues), a central protease-sensitive hinge region (approximately 70 residues) and a C-terminal stable endostatin domain (approximately 180 residues). They also demonstrated that proteolytic release of endostatin can occur through several pathways, which may lead to a switch from a matrix-associated to a more soluble endocrine form.
Related Links	http://dx.doi.org/10.1093/emboj/17.15.4249
Ending Page	4256
Page Count	8
Starting Page	4249
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	15
Volume Number	17
Language	English
Publisher Date	1998-08-03
Access Restriction	Open
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Collagen XVIII/endostatin is essential for vision and retinal pigment epithelial function

Secreted cathepsin L generates endostatin from collagen XVIII

Lack of collagen XVIII/endostatin results in eye abnormalities

Crystal structure of the angiogenesis inhibitor endostatin at 1.5 A resolution.

Structural basis and potential role of heparin/heparan sulfate binding to the angiogenesis inhibitor endostatin.

Crystal structure of an angiogenesis inhibitor bound to the FGF receptor tyrosine kinase domain.

The structure of the C-terminal actin-binding domain of talin

The solution structure of the C-terminal domain of the Mu B transposition protein

Solution structure of a conserved C-terminal domain of p73 with structural homology to the SAM domain.

Structure, function and tissue forms of the C-terminal globular domain of collagen XVIII containing the angiogenesis inhibitor endostatin.

Similar Documents

Collagen XVIII/endostatin is essential for vision and retinal pigment epithelial function

Secreted cathepsin L generates endostatin from collagen XVIII

Lack of collagen XVIII/endostatin results in eye abnormalities

Crystal structure of the angiogenesis inhibitor endostatin at 1.5 A resolution.

Structural basis and potential role of heparin/heparan sulfate binding to the angiogenesis inhibitor endostatin.

Crystal structure of an angiogenesis inhibitor bound to the FGF receptor tyrosine kinase domain.

The structure of the C-terminal actin-binding domain of talin

The solution structure of the C-terminal domain of the Mu B transposition protein

Solution structure of a conserved C-terminal domain of p73 with structural homology to the SAM domain.

Structure, function and tissue forms of the C-terminal globular domain of collagen XVIII containing the angiogenesis inhibitor endostatin.