NDLI: Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays.

Content Provider	PubMed Central
Author	Logie, C. Peterson, C. L.
Abstract	A novel, quantitative nucleosome array assay has been developed that couples the activity of a nucleosome 'remodeling' activity to restriction endonuclease activity. This assay has been used to determine the kinetic parameters of ATP-dependent nucleosome disruption by the yeast SWI/SNF complex. Our results support a catalytic mode of action for SWI/SNF in the absence of nucleosome targeting. In this quantitative assay SWI/SNF and ATP lead to a 100-fold increase in nucleosomal DNA accessibility, and initial rate measurements indicate that the complex can remodel one nucleosome every 4.5 min on an 11mer nucleosome array. In contrast to SWI/SNF action on mononucleosomes, we find that the SWI/SNF remodeling reaction on a nucleosome array is a highly reversible process. This result suggests that recovery from SWI/SNF action involves interactions among nucleosomes. The biophysical properties of model nucleosome arrays, coupled with the ease with which homogeneous arrays can be reconstituted and the DNA accessibility analyzed, makes the described array system generally applicable for functional analysis of other nucleosome remodeling enzymes, including histone acetyltransferases.
Related Links	http://dx.doi.org/10.1093/emboj/16.22.6772
Ending Page	6782
Page Count	11
Starting Page	6772
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	22
Volume Number	16
Language	English
Publisher Date	1997-11-15
Access Restriction	Open
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays.

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Activation domains drive nucleosome eviction by SWI/SNF

The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5.

Continuous and widespread roles for the Swi-Snf complex in transcription.

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Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays.