NDLI: Nuclease hypersensitive regions with adjacent positioned nucleosomes mark the gene boundaries of the PHO5/PHO3 locus in yeast.

Please wait, while we are loading the content...

Year: 2016, Volume: 35

Year: 2015, Volume: 34

Year: 2014, Volume: 33

Year: 2013, Volume: 33

Year: 2013, Volume: 32

Year: 2012, Volume: 32

Year: 2012, Volume: 31

Year: 2011, Volume: 30

Year: 2010, Volume: 29

Year: 2009, Volume: 28

Year: 2008, Volume: 27

Year: 2007, Volume: 26

Year: 2006, Volume: 26

Year: 2006, Volume: 25

Year: 2005, Volume: 24

Year: 2004, Volume: 24

Year: 2004, Volume: 23

Year: 2003, Volume: 22

Year: 2002, Volume: 21

Year: 2001, Volume: 20

Year: 2000, Volume: 19

Year: 1999, Volume: 18

Year: 1998, Volume: 17

Year: 1997, Volume: 16

Year: 1996, Volume: 15

Year: 1995, Volume: 14

Year: 1994, Volume: 13

Year: 1993, Volume: 12

Year: 1992, Volume: 11

Year: 1991, Volume: 10

Year: 1990, Volume: 9

Year: 1989, Volume: 8

Year: 1988, Volume: 7

Year: 1987, Volume: 6

Year: 1986, Volume: 5

Year: 1986, Volume: 5, Issue: 13

Year: 1986, Volume: 5, Issue: 12

Year: 1986, Volume: 5, Issue: 11

Year: 1986, Volume: 5, Issue: 10

Monocot and dicot pre-mRNAs are processed with different efficiencies in transgenic tobacco

Excision of Ds produces waxy proteins with a range of enzymatic activities.

The gene III product (P15) of cauliflower mosaic virus is a DNA-binding protein while an immunologically related P11 polypeptide is associated with virions

Assembly of cyanobacterial and higher plant ribulose bisphosphate carboxylase subunits into functional homologous and heterologous enzyme molecules in Escherichia coli

Pigment—protein interactions in the photosynthetic reaction centre from Rhodopseudomonas viridis

X-ray crystal structure of the complex of human leukocyte elastase (PMN elastase) and the third domain of the turkey ovomucoid inhibitor.

Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP.

The mouse E beta 2 gene: a class II MHC beta gene with limited intraspecies polymorphism and an unusual pattern of transcription.

Alternative processing of H-2Dd pre-mRNAs results in membrane expression of differentially phosphorylated protein products.

Cadherin cell adhesion molecules with distinct binding specificities share a common structure.

Tetanus toxin: primary structure, expression in E. coli, and homology with botulinum toxins.

Insulin-like growth factor I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity.

Human basic fibroblast growth factor: nucleotide sequence and genomic organization.

Structure and expression of cDNA and genes for human interferon-beta-2, a distinct species inducible by growth-stimulatory cytokines.

Endothelial plasminogen activator inhibitor (PAI): a new member of the Serpin gene family.

Identification of centrosomal proteins in a human lymphoblastic cell line.

Beta zero thalassemia caused by a base substitution that creates an alternative splice acceptor site in an intron.

A 5' duplication of the alpha-cardiac actin gene in BALB/c mice is associated with abnormal levels of alpha-cardiac and alpha-skeletal actin mRNAs in adult cardiac tissue.

A domain of SV40 capsid polypeptide VP1 that specifies migration into the cell nucleus.

Purification of a factor specific for the upstream element of the adenovirus-2 major late promoter.

The 3' region of bovine leukemia virus genome encodes a trans-activator protein.

The major polypeptide of scrapie-associated fibrils (SAF) has the same size, charge distribution and N-terminal protein sequence as predicted for the normal brain protein (PrP).

Restricted expression of EBV latent genes and T-lymphocyte-detected membrane antigen in Burkitt's lymphoma cells.

Transformation and stimulation of DNA synthesis in NIH-3T3 cells are a titratable function of normal p21N-ras expression.

Amplification and overexpression of the met gene in spontaneously transformed NIH3T3 mouse fibroblasts.

Stoichiometric association of cap-binding protein I with translated polysomal globin mRNP.

ATP-linked monomer-polymer equilibrium of smooth muscle myosin: the free folded monomer traps ADP.Pi.

Reconstitution of the purified alpha-latrotoxin receptor in liposomes and planar lipid membranes. Clues to the mechanism of toxin action.

Engrailed gene expression in Drosophila imaginal discs.

On the molecular mechanism of gypsy-induced mutations at the yellow locus of Drosophila melanogaster.

Sequence and structure of the dopa decarboxylase gene of Drosophila: evidence for novel RNA splicing variants.

Integrative transformation of Caenorhabditis elegans

Nuclease hypersensitive regions with adjacent positioned nucleosomes mark the gene boundaries of the PHO5/PHO3 locus in yeast.

Removal of positioned nucleosomes from the yeast PHO5 promoter upon PHO5 induction releases additional upstream activating DNA elements.

KIN28, a yeast split gene coding for a putative protein kinase homologous to CDC28.

3'-End formation of transcripts from the yeast rRNA operon.

An intron in a ribosomal protein gene from Tetrahymena

The mom gene of bacteriophage Mu: the mechanism of methylation-dependent expression.

The three-dimensional structure of α1-purothionin in solution: combined use of nuclear magnetic resonance, distance geometry and restrained molecular dynamics

A computer aided thermodynamic approach for predicting the formation of Z-DNA in naturally occurring sequences.

Complete sequence and transcript regulation of a cell adhesion protein from aggregating Dictyostelium cells

In situ hybridization of immunoglobulin-specific RNA in single cells of the B lymphocyte lineage with radiolabelled DNA probes

Year: 1986, Volume: 5, Issue: 9

Year: 1986, Volume: 5, Issue: 8

Year: 1986, Volume: 5, Issue: 7

Year: 1986, Volume: 5, Issue: 6

Year: 1986, Volume: 5, Issue: 5

Year: 1986, Volume: 5, Issue: 4

Year: 1986, Volume: 5, Issue: 3

Year: 1986, Volume: 5, Issue: 2

Year: 1986, Volume: 5, Issue: 1

Year: 1985, Volume: 4

Year: 1984, Volume: 3

Year: 1983, Volume: 2

Year: 1982, Volume: 1

Nuclease hypersensitive regions with adjacent positioned nucleosomes mark the gene boundaries of the PHO5/PHO3 locus in yeast.

Content Provider	PubMed Central
Author	Almer, A. Hörz, W.
Abstract	The chromatin structure of two tandemly linked acid phosphatase genes (PHO5 and PHO3) from Saccharomyces cerevisiae was analyzed under conditions at which the strongly regulated PHO5 gene is repressed. Digestion experiments with DNase I, DNase II, micrococcal nuclease and restriction nucleases reveal the presence of five hypersensitive sites at the PHO5/PHO3 locus, two of them upstream of PHO5 at distances of 920 and 370 bp, one in between the two genes and two downstream of PHO3. Specifically positioned nucleosomes are located next to these hypersensitive sites as shown by indirect end-labeling experiments. The positions deduced from these experiments could be verified by monitoring the accessibility of various restriction sites to the respective nucleases. Sites within putative linker regions were about 50-60% susceptible, whereas sites located within nucleosome cores were resistant. Hybridizing micrococcal nuclease digests to a probe from in between the two upstream hypersensitive sites leads to an interruption of an otherwise regular nucleosomal DNA pattern. This shows directly that these hypersensitive sites represent gaps within ordered nucleosomal arrays.
Starting Page	2681
File Format	PDF
ISSN	14602075
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	10
Volume Number	5
Language	English
Publisher Date	1986-10-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in