NDLI: Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing

Content Provider	PubMed Central
Author	Hui, Jingyi Hung, Lee-hsueh Heiner, Monika Schreiner, Silke Neumüller, Norma Reither, Gregor Haas, Stefan A. Albrecht, Bindereif
Copyright Year	2005
Abstract	We have recently identified an intronic polymorphic CA-repeat region in the human endothelial nitric oxide synthase (eNOS) gene as an important determinant of the splicing efficiency, requiring specific binding of hnRNP L. Here, we analyzed the position requirements of this CA-repeat element, which revealed its potential role in alternative splicing. In addition, we defined the RNA binding specificity of hnRNP L by SELEX: not only regular CA repeats are recognized with high affinity but also certain CA-rich clusters. Therefore, we have systematically searched the human genome databases for CA-repeat and CA-rich elements associated with alternative 5′ splice sites (5′ss), followed by minigene transfection assays. Surprisingly, in several specific human genes that we tested, intronic CA RNA elements could function either as splicing enhancers or silencers, depending on their proximity to the alternative 5′ss. HnRNP L was detected specifically bound to these diverse CA elements. These data demonstrated that intronic CA sequences constitute novel and widespread regulatory elements of alternative splicing.
Related Links	http://dx.doi.org/10.1038/sj.emboj.7600677
Ending Page	1998
Page Count	11
Starting Page	1988
File Format	PDF
ISSN	02614189
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	11
Volume Number	24
Language	English
Publisher	Nature Publishing Group
Publisher Date	2005-05-01
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.

The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements

The intronic splicing code: multiple factors involved in ATM pseudoexon definition

Muscleblind proteins regulate alternative splicing

The splicing regulators Esrp1 and Esrp2 direct an epithelial splicing program essential for mammalian development

Self-splicing of the Tetrahymena intron from mRNA in mammalian cells.

HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment

Intrasplicing coordinates alternative first exons with alternative splicing in the protein 4.1R gene

Processing of intronic microRNAs

Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing

Similar Documents

The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.

The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements

The intronic splicing code: multiple factors involved in ATM pseudoexon definition

Muscleblind proteins regulate alternative splicing

The splicing regulators Esrp1 and Esrp2 direct an epithelial splicing program essential for mammalian development

Self-splicing of the Tetrahymena intron from mRNA in mammalian cells.

HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment

Intrasplicing coordinates alternative first exons with alternative splicing in the protein 4.1R gene

Processing of intronic microRNAs

Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing