NDLI: Cell-Dependent Requirement of Human Immunodeficiency Virus Type 1 gp41 Cytoplasmic Tail for Env Incorporation into Virions

Content Provider	PubMed Central
Author	Akari, Hirofumi Fukumori, Tomoharu Adachi, Akio
Copyright Year	2000
Abstract	Growth kinetics in lymphocytic H9 and M8166 cells of two mutants of human immunodeficiency virus type 1 (HIV-1) with deleted gp41 cytoplasmic tails were examined. While the mutant viruses designated CTdel-44 and CTdel-144 were able to grow in M8166 cells, they were unable to grow in H9 cells. Transfection and single-round infectivity assays demonstrated that they are defective in the early phase of viral replication in H9 cells. Analysis of the mutant virions revealed drastically reduced incorporation of Env gp120 (compared with the incorporation of wild-type virions) in H9 cells but normal incorporation in M8166 cells. These results indicate that the HIV-1 cytoplasmic tail of gp41 determines virus infectivity in a cell-dependent manner by affecting incorporation of Env into virions and suggest the involvement of a host cell factor(s) in the Env incorporation.
Starting Page	4891
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	10
Volume Number	74
Language	English
Publisher	American Society for Microbiology
Publisher Date	2000-05-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

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Cell-Dependent Requirement of Human Immunodeficiency Virus Type 1 gp41 Cytoplasmic Tail for Env Incorporation into Virions