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| Content Provider | PubMed Central |
|---|---|
| Author | Chen, Hsiao Ying Lu, Yang Howard, Teresa Anderson, David Fong, Priscilla Yiquan Hu, Wei-ping Chia, Chee Poh Guan, Ming |
| Copyright Year | 2005 |
| Abstract | An immunochromatographic test for rapid detection of IgM antibodies in patients with acute hepatitis E infection was developed utilizing the well-characterized recombinant protein EP2.1 and monoclonal antibody 4B2. The new rapid test based on a novel reverse-flow technology was able to generate a positive result within 2 to 3 min. Our study showed that this test was able to detect anti-HEV IgM antibodies in 96.7% of the patient samples tested (n = 151) while maintaining an excellent specificity of 98.6% with samples from various patient or healthy control groups (total n = 208). Furthermore, this rapid test gave a good specificity of 90.9% when tested with rheumatoid factor (RF)-positive sera (RF value of ≤850 IU/ml; n = 11) although a higher concentration of RF in samples might cause cross-reactivity. The new test has a good agreement of 97.2% with a kappa value of 0.943 when compared with a reference enzyme-linked immunosorbent assay. The positive predictive value and the negative predictive value for the rapid test thus reached 98.0 and 97.6%, respectively. This is the first rapid, point-of-care test for hepatitis E and will be especially useful for the diagnosis of acute hepatitis E virus infection in field and emergency settings and in resource-poor countries. |
| Related Links | http://dx.doi.org/10.1128/cdli.12.5.593-598.2005 |
| Ending Page | 598 |
| Page Count | 6 |
| Starting Page | 593 |
| File Format | |
| ISSN | 15566811 |
| e-ISSN | 10986588 |
| Journal | Clinical and Diagnostic Laboratory Immunology |
| Issue Number | 5 |
| Volume Number | 12 |
| Language | English |
| Publisher | American Society for Microbiology |
| Publisher Date | 2005-05-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology |
| Subject Keyword | Immunology Immunology and Allergy Microbiology (medical) Clinical Biochemistry Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Clinical Biochemistry Immunology Microbiology (medical) |
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