NDLI: Activation of the BRLF1 promoter and lytic cycle of Epstein

Content Provider	PubMed Central
Author	Chang, Li-kwan Liu, Shih-tung
Copyright Year	2000
Abstract	Histone acetylation alters the chromatin structure and activates the genes that are repressed by histone deacetylation. This investigation demonstrates that treating P3HR1 cells with trichostatin A (TSA) activates the Epstein–Barr virus (EBV) lytic cycle, allowing the virus to synthesize three viral lytic proteins—Rta, Zta and EA-D. Experimental results indicate that TSA and 12-O-tetradecanoylphorbol-13-acetate synergistically activate the transcription of BRLF1, an immediate-early gene of EBV. Chromatin immunoprecipitation assay reveals that histone H4 at the BRLF1 promoter is acetylated after P3HR1 cells are treated with TSA, suggesting that histone acetylation activates BRLF1 transcription. Furthermore, results in this study demonstrate that mutation of a YY1-binding site in the BRLF1 promoter activates BRLF1 transcription 1.6- and 2.3-fold in P3HR1 cells and C33A cells, respectively. Real time PCR analysis reveals that the mutation also increases the histone acetylation level of the nucleosomes at the BRLF1 promoter 1.64- and 3.08-fold in P3HR1 and C33A cells, respectively. Results presented herein suggest that histone deacetylation plays an important role in maintaining the viral latency and histone acetylation at the BRLF1 promoter allows the virus to express Rta and to activate the viral lytic cycle.
Starting Page	3918
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	20
Volume Number	28
Language	English
Publisher	Oxford University Press
Publisher Date	2000-10-15
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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Activation of the BRLF1 promoter and lytic cycle of Epstein–Barr virus by histone acetylation

Direct BRLF1 binding is required for cooperative BZLF1/BRLF1 activation of the Epstein – Barr virus early promoter, BMRF1

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