NDLI: Processing of the Intron-Encoded U18 Small Nucleolar RNA in the Yeast Saccharomyces cerevisiae Relies on Both Exo- and Endonucleolytic Activities

Content Provider	PubMed Central
Author	Villa, Tommaso Francesca, Ceradini Presutti, Carlo Bozzoni, Irene
Copyright Year	1998
Abstract	Many small nucleolar RNAs (snoRNAs) are encoded within introns of protein-encoding genes and are released by processing of their host pre-mRNA. We have investigated the mechanism of processing of the yeast U18 snoRNA, which is found in the intron of the gene coding for translational elongation factor EF-1β. We have focused our analysis on the relationship between splicing of the EF-1β pre-mRNA and production of the mature snoRNA. Mutations inhibiting splicing of the EF-1β pre-mRNA have been shown to produce normal U18 snoRNA levels together with the accumulation of intermediates deriving from the pre-mRNA, thus indicating that the precursor is an efficient processing substrate. Inhibition of 5′→3′ exonucleases obtained by insertion of G cassettes or by the use of a rat1-1 xrn1Δ mutant strain does not impair U18 release. In the Exo− strain, 3′ cutoff products, diagnostic of an endonuclease-mediated processing pathway, were detected. Our data indicate that biosynthesis of the yeast U18 snoRNA relies on two different pathways, depending on both exonucleolytic and endonucleolytic activities: a major processing pathway based on conversion of the debranched intron and a minor one acting by endonucleolytic cleavage of the pre-mRNA.
Starting Page	3376
File Format	PDF
ISSN	10985549
e-ISSN	10985549
Journal	Molecular and Cellular Biology
Issue Number	6
Volume Number	18
Language	English
Publisher	American Society for Microbiology
Publisher Date	1998-06-01
Access Restriction	Open
Rights Holder	American Society for Microbiology
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology

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